Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacký University, tr. 17 listopadu 1192/12, Olomouc, Czech Republic.
Skin Res Technol. 2010 Aug;16(3):365-70. doi: 10.1111/j.1600-0846.2010.00442.x.
BACKGROUND/PURPOSE: All living organisms emit spontaneous ultra-weak photon emission as a result of cellular metabolic processes. Exposure of living organisms to exogenous factors results in oxidative processes and enhancement in ultra-weak photon emission. Here, hydrogen peroxide (H(2)O(2)), as a strongly oxidizing molecule, was used to induce oxidative processes and enhance ultra-weak photon emission in human hand skin. The presented work intends to compare both spontaneous and peroxide-induced ultra-weak photon emission from the epidermal cells on the dorsal and the palm side of the hand.
A highly sensitive photomultiplier tube and a charge-coupled device camera were used to detect ultra-weak photon emission from human hand skin.
Spontaneous ultra-weak photon emission from the epidermal cells on the dorsal side of the hand was 4 counts/s. Topical application of 500 mM H(2)O(2) to the dorsal side of the hand caused enhancement in ultra-weak photon emission to 40 counts/s. Interestingly, both spontaneous and peroxide-induced ultra-weak photon emission from the epidermal cells on the palm side of the hand were observed to increase twice their values, i.e. 8 and 80 counts/s, respectively. Similarly, the two-dimensional image of ultra-weak photon emission observed after topical application of H(2)O(2) to human skin reveals that photon emission from the palm side exceeds the photon emission from the dorsal side of the hand.
The results presented indicate that the ultra-weak photon emission originating from the epidermal cells on the dorsal and the palm side of the hand is related to the histological structure of the human hand skin. Ultra-weak photon emission is shown as a non-destructive technique for monitoring of oxidative processes in the epidermal cells of the human hand skin and as a diagnostic tool for skin diseases.
背景/目的：所有生物体都会因细胞代谢过程而自发地发射超微弱的光子。生物体受到外源性因素的暴露会导致氧化过程，并增强超微弱光子的发射。在这里，过氧化氢（H 2 O 2 ）作为一种强氧化剂，被用于诱导氧化过程，并增强人体手部皮肤表皮细胞的超微弱光子发射。本研究旨在比较手部背部和手掌侧表皮细胞的自发和过氧化物诱导的超微弱光子发射。
使用高灵敏度光电倍增管和电荷耦合器件（CCD）相机来检测人体手部皮肤的超微弱光子发射。
手部背部表皮细胞的自发超微弱光子发射为 4 计数/秒。将 500mM H 2 O 2 局部应用于手部背部，导致超微弱光子发射增强至 40 计数/秒。有趣的是，手部手掌侧的自发和过氧化物诱导的超微弱光子发射均观察到其值增加了两倍，即 8 和 80 计数/秒。同样，将 H 2 O 2 局部应用于人体皮肤后观察到的超微弱光子发射二维图像表明，手掌侧的光子发射超过了手部背部的光子发射。
所呈现的结果表明，手部背部和手掌侧表皮细胞的超微弱光子发射与人体手部皮肤的组织学结构有关。超微弱光子发射是一种非破坏性技术，可用于监测人体手部皮肤表皮细胞的氧化过程，并作为皮肤疾病的诊断工具。
