Guenounou M, Agneray J
C R Acad Hebd Seances Acad Sci D. 1978 Jan;286(1):161-4.
Mouse spleen cells treated with sodium periodate for 10 min. at 4 degrees C are stimulated to undergo blastogenesis and to incorporate thymidine. The effect of such treatment on the antibody response in vitro induced by Sheep red blood cells has been evaluated. Periodate-induced proliferation is accompanied by a marked inhibition of the immune response to this antigen. At concentrations leading to mitogenesis, no cytotoxic effect of periodate was observed and treated cells survived well on tissue culture. Cell recoveries from samples treated with periodate at the optimal mitogenic dose, were markedly enhanced when harvested at different days after culturing wheras lower antibody forming cells numbers wereconsistently observed during the culture period.
在4℃下用高碘酸钠处理10分钟的小鼠脾细胞被刺激发生胚细胞生成并掺入胸腺嘧啶核苷。已经评估了这种处理对绵羊红细胞体外诱导的抗体反应的影响。高碘酸钠诱导的增殖伴随着对该抗原免疫反应的显著抑制。在导致有丝分裂的浓度下，未观察到高碘酸钠的细胞毒性作用，并且处理后的细胞在组织培养中存活良好。当在培养后的不同天数收获时，以最佳有丝分裂剂量用高碘酸钠处理的样品的细胞回收率显著提高，而在培养期间始终观察到较低的抗体形成细胞数量。
