Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Microbes Infect. 2010 Nov;12(12-13):1079-84. doi: 10.1016/j.micinf.2010.06.011. Epub 2010 Jul 15.
The genome of influenza type A virus consists of single-stranded RNAs of negative polarity. Progeny viral RNA (vRNA) replicated in the nucleus is nuclear-exported, and finally transported to the budding site beneath the plasma membrane. However, the precise process of the membrane targeting of vRNA is unclear, although viral proteins and cytoskeleton are thought to play roles. Here, we have visualized the translocation process of progeny vRNA using fluorescence in situ hybridization method. Our results provide an evidence of the involvement of vesicular trafficking in membrane targeting of progeny vRNA independent of that of viral membrane proteins.
甲型流感病毒的基因组由单链负义 RNA 组成。在细胞核中复制的子代病毒 RNA（vRNA）被核输出，并最终运输到质膜下的出芽部位。然而，尽管认为病毒蛋白和细胞骨架起作用，但 vRNA 的膜靶向的精确过程尚不清楚。在这里，我们使用荧光原位杂交方法可视化了子代 vRNA 的易位过程。我们的结果提供了证据，证明囊泡运输参与了子代 vRNA 的膜靶向，而不依赖于病毒膜蛋白。
