College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, Lanzhou University, Lanzhou 730000, China.
J Biotechnol. 2010 Oct 1;150(1):171-4. doi: 10.1016/j.jbiotec.2010.07.013. Epub 2010 Jul 16.
In present study, a facile prepared nano-sized magnetic support was successfully synthesized. Then this support was applied for lipase immobilization using glutaraldehyde as a coupling agent. Experimental data showed that the immobilized lipase exhibited good thermal stability and reusability. The lipase loading amount and activity recovery were found to be 43.6 mg/g support and 58.2%. Kinetic studies suggested it an acceptable degree of specificity retention for an immobilization process.
在本研究中，成功合成了一种制备简便的纳米磁性载体。然后，该载体通过戊二醛作为偶联剂用于脂肪酶的固定化。实验数据表明，固定化脂肪酶表现出良好的热稳定性和可重复使用性。酶的载量和酶活回收率分别为 43.6 mg/g 载体和 58.2%。动力学研究表明，固定化过程对特异性保留具有可接受的程度。
