Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie (LGP), Avenue de Mus de Loup, 17390 La Tremblade, France.
Virus Res. 2010 Oct;153(1):92-9. doi: 10.1016/j.virusres.2010.07.011. Epub 2010 Jul 16.
Ostreid herpesvirus 1 (OsHV-1) infections have been reported around the world and are associated with high mortalities of the Pacific oyster (Crassostrea gigas). In the summer 2008, abnormal mortality rates ranging from 80% to 100% were reported in France and affected only Pacific oysters. Analyses of oyster samples collected during mortality outbreaks demonstrated a significant detection of OsHV-1 (75% of analysed batches), which appeared stronger than previous years. DNA sequencing based on C and IA regions was carried out on 28 batches of OsHV-1 infected Pacific oysters collected in 2008. Polymorphisms were described in both the C and IA regions and characterized a genotype of OsHV-1 not already reported and termed OsHV-1 microVar. A microsatellite zone present in the C region showed several deletions. Additionally, 44 isolates collected in France and in the USA, from 1995 to 2007 were sequenced and compared to the 2008 sequences. The analyses of 76 sequences showed OsHV-1 microVar detection only in 2008 isolates. These data suggest that OsHV-1 microVar can be assumed as an emergent genotype.
牡蛎疱疹病毒 1（OsHV-1）感染在世界各地均有报道，并与太平洋牡蛎（Crassostrea gigas）的高死亡率相关。2008 年夏季，法国报告了死亡率高达 80%至 100%的异常情况，且仅影响太平洋牡蛎。对死亡率爆发期间采集的牡蛎样本的分析表明，显著检测到了 OsHV-1（分析的批次中有 75%），其强度大于往年。对 2008 年采集的 28 批感染 OsHV-1 的太平洋牡蛎进行了基于 C 和 IA 区的 DNA 测序。在 C 和 IA 区均描述了多态性，并确定了一种以前未报道过的 OsHV-1 基因型，称为 OsHV-1 microVar。C 区存在的微卫星区显示出多个缺失。此外，还对 1995 年至 2007 年期间在法国和美国采集的 44 个分离株进行了测序，并与 2008 年的序列进行了比较。对 76 个序列的分析表明，仅在 2008 年的分离株中检测到 OsHV-1 microVar。这些数据表明，OsHV-1 microVar 可以假定为一种新兴的基因型。
