Teltow G J, Fournier P V, Rawlings J A
Microbiological Services Division, Bureau of Laboratories, Texas Department of Health, Austin.
Am J Trop Med Hyg. 1991 May;44(5):469-74. doi: 10.4269/ajtmh.1991.44.469.
The Texas Department of Health Laboratory cultured arthropods from November 1988 through December 1989 in an attempt to isolate Borrelia burgdorferi, the etiologic agent of Lyme disease. Spirochetes were isolated from eight of 1,093 pools of arthropods cultured. The spirochetal isolates were from several tick and one flea species, including Amblyomma americanum, A. maculatum, Ixodes scapularis, and Ctenocephalides felis. These 8 isolates reacted specifically when treated with monoclonal antibodies to B. burgdorferi. Polyacrylamide gel electrophoresis of six lysates showed them to be virtually identical with strain B31 of B. burgdorferi.
1988年11月至1989年12月期间，得克萨斯州卫生部门实验室对节肢动物进行培养，试图分离出莱姆病的病原体——伯氏疏螺旋体。在培养的1093组节肢动物中，有8组分离出了螺旋体。这些螺旋体分离株来自几种蜱虫和一种跳蚤，包括美洲钝眼蜱、黄斑钝眼蜱、肩突硬蜱和猫栉首蚤。用针对伯氏疏螺旋体的单克隆抗体处理时，这8株分离株产生特异性反应。六种裂解物的聚丙烯酰胺凝胶电泳显示，它们与伯氏疏螺旋体B31菌株几乎相同。
