用于高光谱显微镜的具有高采样密度的快照图像映射光谱仪（IMS）。
Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.
作者信息
Gao Liang, Kester Robert T, Hagen Nathan, Tkaczyk Tomasz S
机构信息
Department of Bioengineering, Rice University, Houston, TX, 77005, USA.
出版信息
Opt Express. 2010 Jul 5;18(14):14330-44. doi: 10.1364/OE.18.014330.
Abstract
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.
摘要
一种具有高采样密度的快照图像映射光谱仪（IMS）被开发用于高光谱显微镜，测量尺寸为285×285×60（x、y、λ）的数据立方体。空间分辨率约为0.45微米，视场为100×100微米²。测量光谱范围为450纳米至650纳米，由60个光谱通道采样，平均采样间隔约为3.3纳米。通道的光谱分辨率约为8纳米。光谱成像结果证明了IMS用于实时细胞荧光成像的潜力。
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