Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, People's Republic of China.
Biotechnol Lett. 2010 Dec;32(12):1765-75. doi: 10.1007/s10529-010-0356-z. Epub 2010 Jul 18.
Cellulases hydrolyze the β-1,4 linkages of cellulose and are widely used in food, brewing and wine, animal feed, textiles and laundry, and pulp and paper industries, especially for hydrolyzing cellulosic materials into sugars, which can be fermented to produce useful products such as ethanol. Metagenomics has become an alternative approach to conventional culture-dependent methods as it allows exhaustive mining of microbial genomes in their natural environments. This review covers the current state of research and challenges in mining novel cellulase genes from the metagenomes of various environments, and discusses the potential biotechnological applications of metagenome-derived cellulases.
纤维素酶水解纤维素的β-1,4 键，广泛应用于食品、酿造和葡萄酒、动物饲料、纺织和洗衣以及纸浆和造纸行业，特别是用于将纤维素材料水解成糖，这些糖可以发酵生产有用的产品，如乙醇。宏基因组学已成为传统基于培养的方法的替代方法，因为它可以彻底挖掘微生物在其自然环境中的基因组。本文综述了从各种环境的宏基因组中挖掘新型纤维素酶基因的研究现状和挑战，并讨论了宏基因组衍生纤维素酶的潜在生物技术应用。
