Balejová Magda
Central Laboratories, The Laboratory of Bacteriology, Ceské Budejovice, Czech Republic.
Klin Mikrobiol Infekc Lek. 2010 Jun;16(3):80-5.
Preliminary identification of clinically significant Clostridium spp. is based on evaluating their microscopic and macroscopic morphology, Gram staining (Gram stain-positive structure of the bacterial wall), positive production of lecithinase, lipase and proteolytic activity on egg yolk agar, and simple chemical tests. If this preliminary identification is not sufficient, biochemical identification is performed, along with 16S-rRNA sequencing of the bacterial genome. The article comments on options of preliminary identification of clinically significant Clostridium spp.
临床上重要梭菌属细菌的初步鉴定基于对其微观和宏观形态、革兰氏染色(细菌细胞壁革兰氏染色阳性结构)、卵磷脂酶阳性产生、在卵黄琼脂上的脂肪酶和蛋白水解活性以及简单化学试验的评估。如果这种初步鉴定不充分,则进行生化鉴定以及细菌基因组的16S-rRNA测序。本文评论了临床上重要梭菌属细菌初步鉴定的方法。