Wageningen UR Food & Biobased Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
J Control Release. 2010 Oct 15;147(2):298-303. doi: 10.1016/j.jconrel.2010.07.098. Epub 2010 Jul 17.
We studied the release of entrapped protein from transient gels made of thermosensitive, collagen-inspired ABA triblock copolymers with tailorable properties and with mid blocks of two different lengths (~37 kDa and ~73 kDa). These polymers were produced as heterologous proteins in recombinant yeast. By varying polymer length and concentration, the elastic properties of the hydrogels as well as their mesh size, swelling and erosion could be tuned. Whereas the volume of the investigated dense networks decreased in time as a result of temperature- and polymer concentration-dependent surface erosion, the release of entrapped protein was governed by a combination of gel erosion and protein diffusion. The prevalence of one or the other was strongly dependent on polymer concentration. Most importantly, the encapsulated protein was quantitatively released, which demonstrates that these hydrogels offer great potential as drug delivery systems.
我们研究了热敏感、受胶原启发的 ABA 三嵌段共聚物瞬态凝胶中包埋蛋白质的释放情况，这些共聚物具有可调节的特性，且中间嵌段的长度不同（37 kDa 和73 kDa）。这些聚合物作为重组酵母中的异源蛋白进行生产。通过改变聚合物的长度和浓度，可以调节水凝胶的弹性特性以及其网格大小、溶胀和侵蚀。尽管由于温度和聚合物浓度依赖性的表面侵蚀，所研究的致密网络的体积随时间减少，但包埋蛋白质的释放受凝胶侵蚀和蛋白质扩散的共同作用控制。一种或另一种情况的占主导地位强烈依赖于聚合物浓度。最重要的是，包埋的蛋白质被定量释放，这表明这些水凝胶作为药物传递系统具有很大的潜力。
