Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, No 17 Changlexi Road, Xi'an 710032, People's Republic of China.
Neurosci Lett. 2010 Oct 8;483(1):1-5. doi: 10.1016/j.neulet.2010.07.027. Epub 2010 Jul 17.
It is known that oxidative stress plays a major role in the progression of Parkinson's disease (PD). Previous studies have suggested that 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (TSG), an active component extracted from a traditional Chinese herb Polygonum multiflorum Thunb., has significant antioxidant and free radical-scavenging activities. This is the first study that investigated the protective effects of TSG against MPP(+)-induced apoptosis in PC12 cells and determined the underlying mechanism. The results showed that incubation of PC12 cells with TSG before exposing them to MPP(+) could significantly decrease cell viability loss and reverse cell apoptosis in a dose-dependent manner. The anti-apoptotic effects of TSG were probably mediated via the inhibition of ROS generation and modulation of JNK activation because TSG blocked ROS increase and JNK phosphorylation induced by MPP(+). Taken together, these results indicated that TSG may provide a useful therapeutic strategy for the treatment of neurodegenerative diseases such as PD.
已知氧化应激在帕金森病（PD）的进展中起着重要作用。先前的研究表明，2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG），一种从传统中药何首乌中提取的活性成分，具有显著的抗氧化和自由基清除活性。这是第一项研究，研究了 TSG 对 MPP (+)诱导的 PC12 细胞凋亡的保护作用，并确定了其潜在机制。结果表明，在将 PC12 细胞暴露于 MPP (+)之前用 TSG 孵育，可以显著降低细胞活力损失，并呈剂量依赖性方式逆转细胞凋亡。TSG 的抗凋亡作用可能是通过抑制 ROS 的产生和调节 JNK 的激活来介导的，因为 TSG 阻断了 MPP (+)诱导的 ROS 增加和 JNK 磷酸化。总之，这些结果表明 TSG 可能为治疗 PD 等神经退行性疾病提供一种有用的治疗策略。
