Department of Chemistry, Washington University in Saint Louis, Saint Louis, MO 63130, USA.
Nanoscale. 2010 Apr;2(4):548-58. doi: 10.1039/b9nr00304e. Epub 2010 Feb 8.
Fluorescent cross-linked nanoparticles with variable fluorophore loading amounts, locations, and particle sizes were synthesized from sequential one-pot functionalization/cross-linking of block copolymer micelles with amine-terminated dye and cross-linker molecules, via reductive amination and amidation. The fluorescence quantum yield and brightness of these nanoparticles were evaluated by steady-state and dynamic fluorescence methods. The results demonstrate that the quantum yield and brightness of the fluorescent nanoparticles correlated directly with the number of dyes/nanoparticle and the nanoparticle size. A strategy to increase the fluorescence brightness of nanoparticles with fluorescein and near-infrared dyes is proposed.
荧光交联纳米粒子具有可变的荧光团负载量、位置和粒径，是通过胺封端染料和交联剂分子与嵌段共聚物胶束的顺序一锅法功能化/交联，通过还原胺化和酰胺化反应合成的。通过稳态和动态荧光法评估了这些纳米粒子的荧光量子产率和亮度。结果表明，荧光纳米粒子的量子产率和亮度与染料/纳米粒子的数量和纳米粒子的尺寸直接相关。提出了一种用荧光素和近红外染料提高纳米粒子荧光亮度的策略。
