Takimoto T, Tanaka S, Ishikawa S, Masuda K, Umeda R
Department of Otolaryngology, School of Medicine, Kanazawa University, Japan.
Laryngoscope. 1989 Jul;99(7 Pt 1):741-3. doi: 10.1288/00005537-198907000-00015.
Differences in tumorigenicity between the Epstein-Barr virus (EBV) genome-negative and -positive epithelial A2L/AH hybrid cell lines have been described recently. Further differences in growth characteristics of the two epithelial hybrid cells have since been examined. Differences were found in doubling time and cloning efficiency in agarose of the two cell lines. In addition, the EBV genome-negative epithelial hybrid cell line (cl-654), unlike the EBV genome-positive epithelial hybrid cell line (cl-1), failed to grow in the unmanipulated nude mice, but was successfully transplanted in 60Co-irradiated nude mice. The histopathologic findings of tumors induced by the inoculation of cl-654 cells in nude mice showed more differentiated carcinoma than the findings of tumors induced by cl-1 cells. The data suggest that EBV is associated with an alteration of cell differentiation in epithelial cells as well as tumorigenicity in nude mice.
最近已描述了爱泼斯坦-巴尔病毒（EBV）基因组阴性和阳性的上皮A2L/AH杂交细胞系在致瘤性方面的差异。此后，又对这两种上皮杂交细胞的生长特性的进一步差异进行了研究。发现这两种细胞系在倍增时间和琼脂糖中的克隆效率方面存在差异。此外，EBV基因组阴性的上皮杂交细胞系（cl-654）与EBV基因组阳性的上皮杂交细胞系（cl-1）不同，在未处理的裸鼠中无法生长，但在经60Co照射的裸鼠中成功移植。将cl-654细胞接种到裸鼠中诱导产生的肿瘤的组织病理学结果显示，其癌的分化程度高于cl-1细胞诱导产生的肿瘤的结果。数据表明，EBV与上皮细胞的细胞分化改变以及裸鼠中的致瘤性有关。
