Department of Clinical Pharmacology, Pharmacy Care Center, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Pharm Biol. 2010 Jul;48(7):828-33. doi: 10.3109/13880200903283707.
The constituents of the ethanol extract from the root of Polygala tenuifolia Willd. (Polygalaceae) were investigated for antioxidant activity in senescence-accelerated mice. Consequently, two relevant samples were obtained, a fraction separated by macroporous resin (YZ-OE), and a major pure crystal of 3,6'-disinapoyl sucrose (DISS). Based on HPLC-ESI-MS analysis, the most constituents in the YZ-OE fraction from the extract of P. tenuifolia were oligosaccharide esters. The antioxidant activities of these two samples were evaluated using the accelerated senescence-prone, short-lived mice (SAMP) in vivo. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were increased significantly in SAMP mice fed oligosaccharide esters (YZ-OE 50 mg/kg) and its constituents (DISS 50 mg/kg). However, the content of malondialdehyde (MDA) was increased in the blood and liver of SAMP mice. But when given YZ-OE, it could be decreased, by 44.3% and 47.5%, respectively, compared with the SAMP model. Results from the analyses indicated that the oligosaccharide esters (YZ-OE) from roots of P. tenuifolia had a high in vivo antioxidant activity.
从远志根（远志科）的乙醇提取物中分离的成分，用于研究在快速衰老模型小鼠中的抗氧化活性。结果得到了两个相关的样本，一个是大孔树脂分离的馏分（YZ-OE），另一个是 3,6′-二咖啡酰基蔗糖（DISS）的主要纯晶体。根据 HPLC-ESI-MS 分析，从远志根提取物的 YZ-OE 馏分中含有最多的成分是寡糖酯。使用快速衰老敏感、寿命短的小鼠（SAMP）体内模型评估了这两种样品的抗氧化活性。超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）的活性在喂食寡糖酯（YZ-OE 50mg/kg）及其成分（DISS 50mg/kg）的 SAMP 小鼠中显著增加。然而，SAMP 小鼠血液和肝脏中的丙二醛（MDA）含量增加。但是，给予 YZ-OE 后，与 SAMP 模型相比，血液和肝脏中的 MDA 含量分别降低了 44.3%和 47.5%。分析结果表明，远志根中的寡糖酯（YZ-OE）具有很高的体内抗氧化活性。
