Park Hyun-Ju, Lee Kiwon, Heo Hwon, Lee Myoungsun, Kim Jong Woo, Whang Wei Wan, Kwon Yunhee Kim, Kwon Hyockman
Department of Bioscience and Biotechnology and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies, Yongin 449-791, Korea.
Phytother Res. 2008 Oct;22(10):1324-9. doi: 10.1002/ptr.2488.
Neurogenesis persists in the adult mammalian brain and can be a target for modulation for therapeutic purposes. This study investigated the effect of a Polygala tenuifolia root extract on the proliferation of a stem cell population in the rat hippocampus. The root extract of P. tenuifolia (2 mg/kg/day, 14 times intraperitoneal injections) increased the incorporation of bromodeoxyuridine (BrdU) into cells in the hippocampal CA1 region. This activity was enriched in the saponin-containing fraction. The majority of cells labelled with BrdU were immunoreactive to nestin or Tuj1 and the percentages of nestin/BrdU- and Tuj1/BrdU-double positive cells were increased by the P. tenuifolia root extract, suggesting that the P. tenuifolia root extract promotes the proliferation of neural stem cells. In addition, this extract promoted the neurite outgrowth of rat neuronal precursor cells, HiB5. These activities of P. tenuifolia root extract may contribute to the therapeutic benefits of herbal medicines containing P. tenuifolia root for the treatment of patients with insomnia, neurosis and dementia.
神经发生在成年哺乳动物大脑中持续存在，并且可以成为用于治疗目的的调控靶点。本研究调查了远志根提取物对大鼠海马区干细胞群体增殖的影响。远志根提取物（2毫克/千克/天，腹腔注射14次）增加了溴脱氧尿苷（BrdU）掺入海马CA1区细胞的量。这种活性在含皂苷的组分中富集。大多数用BrdU标记的细胞对巢蛋白或Tuj1具有免疫反应性，远志根提取物增加了巢蛋白/BrdU和Tuj1/BrdU双阳性细胞的百分比，表明远志根提取物促进神经干细胞的增殖。此外，该提取物促进了大鼠神经元前体细胞HiB5的神经突生长。远志根提取物的这些活性可能有助于含远志根的草药对失眠、神经症和痴呆患者治疗的有益效果。
