Department of Biochemistry, Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong SAR, China.
Pharm Biol. 2010 May;48(5):512-7. doi: 10.3109/13880200903190985.
Herba Cistanche, a Chinese herb derived from the whole plant of Cistanche deserticola Y.C. Ma (Orobanchaceae), has been shown to enhance mitochondrial ATP generation and to protect against myocardial ischemia/reperfusion (I/R) injury ex vivo in rats. To define the role of mitochondria in the cardioprotective action of Herba Cistanche, we investigated the effect of Herba Cistanche treatment on mitochondrial glutathione status and functional parameters in rat hearts. Treatment with a methanol extract of Herba Cistanche enhanced mitochondrial glutathione status, decreased mitochondrial Ca(2+) content, and increased mitochondrial membrane potential. In addition, an increase in State 4 respiration, indicative of uncoupled respiration, was observed in mitochondria isolated from Herba Cistanche-treated rat hearts. The enhancement of mitochondrial glutathione status and functional ability, as well as the putative induction of uncoupling proteins, may be related to cardioprotection afforded by Herba Cistanche treatment protecting against I/R injury.
肉苁蓉，一种源自列当科植物管花肉苁蓉（Orobanche deserticola Y.C. Ma）全草的中药，已被证明可增强大鼠心肌线粒体中三磷酸腺苷（ATP）的生成，并可防止心肌缺血/再灌注（I/R）损伤。为了明确肉苁蓉在其心脏保护作用中的线粒体作用，我们研究了肉苁蓉处理对大鼠心脏中线粒体谷胱甘肽状态和功能参数的影响。肉苁蓉甲醇提取物处理可增强线粒体谷胱甘肽状态，降低线粒体钙含量，并增加线粒体膜电位。此外，还观察到肉苁蓉处理的大鼠心脏线粒体中出现呼吸状态 4 增加的现象，表明解偶联呼吸。线粒体谷胱甘肽状态和功能能力的增强，以及可能诱导解偶联蛋白的产生，可能与肉苁蓉处理对 I/R 损伤的心脏保护作用有关。
