Division of Life Science, Hong Kong University of Science & Technology Clear Water Bay, Hong Kong SAR, China.
Pharm Biol. 2013 Jan;51(1):64-73. doi: 10.3109/13880209.2012.710242. Epub 2012 Oct 5.
Earlier findings demonstrated that pretreatment of Herba Cistanches [the dried whole plant of Cistanche deserticola Y.C. Ma (Orobanchaceae)], a "Yang-invigorating" Chinese tonic herb, stimulated the ATP-generation capacity (ATP-GC) in mitochondria isolated from rat heart ex vivo. The enhancement of mitochondrial ATP-GC by Herba Cistanches was associated with induction of glutathione antioxidant status and protection against ischemia/reperfusion (I/R) injury in rat hearts.
This study investigated the relationship between enhancements in mitochondrial ATP-GC and glutathione antioxidant status in H9c2 cardiomyocytes using a semipurified fraction of Herba Cistanches (HCF1).
HCF1 (10-300 ng/mL) was tested for its effects on mitochondrial ATP generation, glutathione antioxidant status and protection against oxidant injury in H9c2 cardiomyocytes and rat hearts.
HCF1 at 30 ng/mL increased mitochondrial ATP-GC and ADP-stimulated state 3 respiration (by 50 and 100%, respectively) in H9c2 cardiomyocytes. The stimulation of mitochondrial respiration was associated with the induction of mitochondrial uncoupling (27%) and enhancement of cellular glutathione redox cycling as well as protection against hypoxia/reoxygenation (hypox/reoxy)-induced apoptosis (by 60%). While HCF1 treatment increased reactive oxygen species generation from mitochondrial respiration in H9c2 cardiomyocytes, pretreatment with antioxidants (DMTU) abrogated the HCF1-induced cellular responses and the associated cytoprotective effect. HCF1 pretreatment (1.14 and 3.41 mg/kg × 14) also protected against myocardial I/R injury in rats (by 13 and 32%), presumably mediated by the induction of glutathione antioxidant response.
The long-term intake of HCF1 may offer a prospect for the prevention of ischemic heart disease.
先前的研究结果表明，预处理肉苁蓉（列当科肉苁蓉的干燥全草）这种“壮阳”的中药可刺激离体大鼠心脏线粒体的三磷酸腺苷生成能力（ATP-GC）。肉苁蓉增强线粒体 ATP-GC 的作用与诱导谷胱甘肽抗氧化状态和保护大鼠心脏免受缺血/再灌注（I/R）损伤有关。
本研究使用肉苁蓉半纯化提取物（HCF1）研究了 H9c2 心肌细胞中线粒体 ATP-GC 和谷胱甘肽抗氧化状态增强之间的关系。
检测了 HCF1（10-300ng/mL）对 H9c2 心肌细胞中线粒体 ATP 生成、谷胱甘肽抗氧化状态和对抗氧化剂损伤的保护作用。
HCF1 在 30ng/mL 时可使 H9c2 心肌细胞中线粒体 ATP-GC 增加 50%，ADP 刺激的第三呼吸状态增加 100%。呼吸的刺激与线粒体解偶联（增加 27%）以及细胞内谷胱甘肽氧化还原循环的增强以及对缺氧/复氧（hypox/reoxy）诱导的细胞凋亡的保护（增加 60%）有关。虽然 HCF1 处理增加了 H9c2 心肌细胞中线粒体呼吸产生的活性氧，但抗氧化剂（DMTU）预处理可消除 HCF1 诱导的细胞反应及其相关的细胞保护作用。HCF1 预处理（1.14 和 3.41mg/kg×14）也可保护大鼠心肌 I/R 损伤（分别减少 13%和 32%），这可能是通过诱导谷胱甘肽抗氧化反应介导的。
长期摄入 HCF1 可能为预防缺血性心脏病提供一种前景。
