Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, Besevler, Ankara, Turkey.
Pharm Biol. 2010 Mar;48(3):253-9. doi: 10.3109/13880200903085482.
Honey-bee pollen mix (HBM) formulation is claimed to be effective for the treatment of asthma, bronchitis, cancers, peptic ulcers, colitis, various types of infections including hepatitis B, and rheumatism by the herb dealers in northeast Turkey. In the present study, in vivo antinociceptive, anti-inflammatory, gastroprotective and antioxidant effects of pure honey and HBM formulation were evaluated comparatively. HBM did not show any significant gastroprotective activity in a single administration at 250 mg/kg dose, whereas a weak activity was observed after three days of successive administration at 500 mg/kg dose. On the other hand, HBM displayed significant antinociceptive (p <0.01) and anti-inflammatory (p <0.01) activities at 500 mg/kg dose orally without inducing any apparent acute toxicity or gastric damage. HBM was also shown to possess potent antilipidperoxidant activity (p <0.01) at 500 mg/kg dose against acetaminophen-induced liver necrosis model in mice. On the other hand, pure honey did not exert any remarkable antinociceptive, anti-inflammatory and gastroprotective activity, but a potent antilipidperoxidant activity (p <0.01) was determined. Results have clearly proved that mixing pure honey with bee pollen significantly increased the healing potential of honey and provided additional support for its traditional use. Total phenolic and flavonoid contents of HBM were found to be 145 and 59.3 mg/100 g of honey, which were estimated as gallic acid and quercetin equivalents, respectively.
土耳其东北部的草药商人声称，蜂蜜花粉混合物（HBM）配方对治疗哮喘、支气管炎、癌症、胃溃疡、结肠炎、乙型肝炎和风湿等疾病有效。在本研究中，比较了纯蜂蜜和 HBM 配方的体内镇痛、抗炎、胃保护和抗氧化作用。HBM 在 250mg/kg 剂量单次给药时没有显示出任何显著的胃保护活性，而在 500mg/kg 剂量连续给药三天后观察到了较弱的活性。另一方面，HBM 在 500mg/kg 剂量下口服显示出显著的镇痛（p<0.01）和抗炎（p<0.01）活性，而没有引起明显的急性毒性或胃损伤。HBM 还显示出在 500mg/kg 剂量下对乙酰氨基酚诱导的小鼠肝坏死模型具有强大的抗脂质过氧化活性（p<0.01）。另一方面，纯蜂蜜没有表现出任何显著的镇痛、抗炎和胃保护活性，但确定了强大的抗脂质过氧化活性（p<0.01）。结果清楚地证明，将纯蜂蜜与蜂花粉混合可显著提高蜂蜜的愈合潜力，并为其传统用途提供了额外的支持。HBM 的总酚和类黄酮含量分别为 145 和 59.3mg/100g 蜂蜜，分别相当于没食子酸和槲皮素当量。
