Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
Pharm Biol. 2010 Mar;48(3):337-41. doi: 10.3109/13880200903133837.
The ethyl acetate and methanol extracts prepared from the fruits, aerial parts, and roots of Heptaptera anatolica (Boiss.) Tutin, (Umbelliferae), H. anisoptera (DC.) Tutin, H. cilicica (Boiss. & Balansa) Tutin (endemic), and H. triquetra (Vent.) Tutin were tested for their acetylcholinesterase (AChE) inhibitory and antioxidant activities. AChE inhibition was evaluated using ELISA microplate reader at 500, 1000, and 2000 microg mL(-1). Antioxidant activity was determined by 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test and Fe(+2)-ferrozine test system for metal chelating power at the same concentrations. Total phenol contents of the extracts were determined using Folin-Ciocalteu reagent. At 2000 microg mL(-1), only the aerial parts and fruits of H. anatolica showed moderate anti-AChE effect (61.97% and 49.80%, respectively), while the aerial parts and fruits of H. triquetra had the highest DPPH scavenging effect (80.48% and 86.19%, respectively). All of the methanol extracts exhibited significant ferrous ion-chelating effect varying between 72.97% and 92.36%, whereas only four of the ethyl acetate extracts exerted chelating effect over 70%. These results indicate that Heptaptera species could be a good source for antioxidant compounds.
从 Heptaptera anatolica（Boiss.） Tutin（伞形科）、H. anisoptera（DC.） Tutin、H. cilicica（Boiss. & Balansa） Tutin（特有种）和 H. triquetra（Vent.） Tutin 的果实、地上部分和根部分别制备的乙酸乙酯和甲醇提取物，经测试具有乙酰胆碱酯酶（AChE）抑制和抗氧化活性。在 500、1000 和 2000μg mL(-1)下使用 ELISA 微量板读取器评估 AChE 抑制作用。抗氧化活性通过 2,2-二苯基-2-苦基肼（DPPH）自由基清除试验和同一浓度下的 Fe(+2)-菲啰嗪试验系统测定金属螯合能力。使用 Folin-Ciocalteu 试剂测定提取物中的总酚含量。在 2000μg mL(-1)下，仅 H. anatolica 的地上部分和果实表现出中度的抗 AChE 作用（分别为 61.97%和 49.80%），而 H. triquetra 的地上部分和果实具有最高的 DPPH 清除作用（分别为 80.48%和 86.19%）。所有甲醇提取物均表现出显著的亚铁离子螯合作用，范围在 72.97%至 92.36%之间，而只有四种乙酸乙酯提取物的螯合作用超过 70%。这些结果表明，Heptaptera 物种可能是抗氧化化合物的良好来源。
