Orhan Ilkay Erdogan, Şener Bilge, Musharraf Syed Ghulam
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
Exp Toxicol Pathol. 2012 Mar;64(3):205-9. doi: 10.1016/j.etp.2010.08.007. Epub 2010 Sep 9.
Fumaria species (Fumariaceae) have been recorded to be used traditionally against liver-related disorders in many countries including Turkey. Oxidative stress is also known to be strongly associated with hepatic problems. Consequently, in the current study, the ethanol extracts of four Fumaria species; F. cilicica Hausskn., F. densiflora DC., F. kralikii Jordan and F. parviflora Lam. growing in Turkey were initially screened for their in vitro antioxidant activities by three methods; 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test, Fe(+2)-ferrozine test system for metal chelating test and ferric-ion reducing antioxidant power (FRAP) at 250, 500 and 1000 μg/ml concentrations. Then, each of the ethanol extracts was fractionated into petroleum ether, chloroform, ethyl acetate and methanol fractions and their antioxidant activities were estimated by DPPH radical scavenging and xanthine oxidase inhibition tests at 1000 μg/ml. In both tests, the chloroform and ethyl acetate fractions of F. cilicica were found to be the most active and were further investigated in in vivo hepatoprotective activity experiment against toxicity induced by CCl(4). Total phenol and flavonoid quantities of the ethanol extracts were determined spectrophotometrically using Folin-Ciocalteau's and AlCl(3) reagents, respectively. Our data revealed that the chloroform and ethyl acetate fractions of F. cilicica did not have hepatoprotective effect and the ethanol extracts exerted low antioxidant activity. Although protective effect of some Fumaria species in hepatic diseases was shown in several previous studies, this record seems to be not pertinent for F. cilicica.
在包括土耳其在内的许多国家，传统上已记载紫堇属植物（罂粟科）可用于治疗与肝脏相关的疾病。氧化应激也被认为与肝脏问题密切相关。因此，在本研究中，首先通过三种方法对生长于土耳其的四种紫堇属植物；细叶紫堇（Fumaria cilicica Hausskn.）、密花紫堇（F. densiflora DC.）、克拉利克紫堇（F. kralikii Jordan）和小花紫堇（F. parviflora Lam.）的乙醇提取物进行体外抗氧化活性筛选；2,2-二苯基-1-苦基肼（DPPH）自由基清除试验、用于金属螯合试验的Fe(+2)-亚铁嗪试验系统以及在250、500和1000μg/ml浓度下的铁离子还原抗氧化能力（FRAP）试验。然后，将每种乙醇提取物分别分离为石油醚、氯仿、乙酸乙酯和甲醇馏分，并通过DPPH自由基清除试验和黄嘌呤氧化酶抑制试验在1000μg/ml浓度下评估其抗氧化活性。在这两种试验中，发现细叶紫堇的氯仿和乙酸乙酯馏分活性最高，并在针对四氯化碳诱导的毒性的体内肝脏保护活性实验中进一步研究。分别使用福林-酚试剂和三氯化铝试剂通过分光光度法测定乙醇提取物中的总酚和黄酮含量。我们的数据显示，细叶紫堇的氯仿和乙酸乙酯馏分没有肝脏保护作用，且乙醇提取物的抗氧化活性较低。尽管先前的几项研究表明某些紫堇属植物对肝脏疾病有保护作用，但这一记录似乎不适用于细叶紫堇。
