Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Hospital Affiliated to Tongji University, Shanghai, China.
Pharm Biol. 2010 Feb;48(2):161-5. doi: 10.3109/13880200903062630.
The aim of the study was to explore the role of basic fibroblast growth factor (bFGF) in ovarian cancer progression. This was done by investigating the effects of bFGF on both the secretion of urokinase-type plasminogen activator (uPA) and the invasion of tumor cells in SKOV(3) ovarian cancer cells. Human ovarian cancer cell line SKOV(3) was cultured in vitro. The expression of uPA gene and protein was induced in SKOV(3) cells; the impact of bFGF on the expression of uPA gene in SKOV(3) cells was studied by RT-PCR, and the impact of bFGF on the expression of uPA protein was tested by ELISA. Ets-1 antisense oligonucleotides were transfected into SKOV(3) cells by liposome protocol. The effects of bFGF on Ets-1 expression and the invasion ability of SKOV(3) cells were determined both before and after exposure to different concentrations of bFGF for 24 h. The expression of both uPA gene and protein was induced in SKOV(3) cells, p < 0.05. The expression of uPA was suppressed by Ets-1 antisense oligonucleotides in SKOV(3) cells, p < 0.05. The invasion ability of SKOV(3) cells was increased by 2.3-fold, and this effect was also suppressed by Ets-1 antisense oligonucleotides. bFGF can enhance the invasion ability of ovarian cancer cells in vitro by inducing the expression of uPA, and this effect is also regulated by the transcription factor Ets-1.
本研究旨在探讨碱性成纤维细胞生长因子(bFGF)在卵巢癌进展中的作用。通过研究 bFGF 对 SKOV(3)卵巢癌细胞 uPA 分泌和侵袭的影响来实现这一目标。SKOV(3)卵巢癌细胞系在体外培养。诱导 SKOV(3)细胞 uPA 基因和蛋白的表达；通过 RT-PCR 研究 bFGF 对 SKOV(3)细胞 uPA 基因表达的影响，通过 ELISA 检测 bFGF 对 SKOV(3)细胞 uPA 蛋白表达的影响。脂质体法将 Ets-1 反义寡核苷酸转染至 SKOV(3)细胞。分别用不同浓度 bFGF 孵育 24 小时前后，观察 bFGF 对 SKOV(3)细胞 Ets-1 表达和侵袭能力的影响。SKOV(3)细胞 uPA 基因和蛋白的表达均被诱导，p<0.05。Ets-1 反义寡核苷酸抑制 SKOV(3)细胞 uPA 的表达，p<0.05。SKOV(3)细胞的侵袭能力增加了 2.3 倍，Ets-1 反义寡核苷酸也抑制了这种作用。bFGF 可通过诱导 uPA 的表达增强卵巢癌细胞在体外的侵袭能力，而这种作用也受转录因子 Ets-1 的调节。
