Mikhaĭlov A M, Nikitenko A V, Chetverina E V, Trakhanov S D, Vaĭnshten B K
Bioorg Khim. 1991 Mar;17(3):372-8.
The structure of the alpha-carbon chain was solved by molecular replacement method at 2.7 A resolution. Neurotoxin I (NTX-I) is one of the main protein components purified from the venom of the central asian cobra Naja naja oxiana. NTX-I is known to bind specifically to acetylcholine receptors thus preventing the transmission of the neuroconductivity signal from synapses to muscles. NTX-I crystals were grown either by vapour diffusion or dialysis methods using specially prepared microdialysis cell. The intensities of reflections from native NTX-I crystals were measured in the range of 38.0-2.1 A-1 by omega-scan method with a Syntex P21 diffractometer operated in automatic regime. To determine the position and mode of packing of NTX-I molecule in unit cell program packages MERLOT and BLANC were applied running on a NORD-500 computer.
通过分子置换法在2.7埃分辨率下解析了α-碳链的结构。神经毒素I（NTX-I）是从中亚眼镜蛇（Naja naja oxiana）毒液中纯化得到的主要蛋白质成分之一。已知NTX-I能特异性结合乙酰胆碱受体，从而阻止神经传导信号从突触传递到肌肉。NTX-I晶体通过蒸汽扩散或透析方法，使用特制的微透析池生长。使用自动运行的Syntex P21衍射仪，通过ω扫描法在38.0 - 2.1 Å⁻¹范围内测量了天然NTX-I晶体的反射强度。为了确定NTX-I分子在晶胞中的位置和堆积方式，应用了在NORD-500计算机上运行的MERLOT和BLANC程序包。
