中亚眼镜蛇毒液中神经毒素-I的结晶及初步X射线衍射研究。

Crystallization and preliminary x-ray diffraction study of neurotoxin-I from Naja naja oxiana venom.

作者信息

Mikhailov A M, Nickitenko A V, Trakhanov S D, Vainshtein B K, Chetverina E V

机构信息

Institute of Crystallography of the USSR Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1990 Aug 20;269(1):255-7. doi: 10.1016/0014-5793(90)81167-m.

DOI:10.1016/0014-5793(90)81167-m

PMID:2387409

Abstract

Crystals of the neurotoxin-I (NTX-I) from the venom of the middle Asian cobra Naja naja oxiana have been grown by vapour diffusion and dialysis methods. The crystals belong to space group P2(1)2(1)2 with dimension of a = 25.19 A, b = 75.59 A, c = 36.09 A and diffract to 1.9 A resolution. The asymmetric unit contains one molecule (Vm = 2.2 A/Da). Using the molecule of alpha-cobratoxin (CTX) as a starting model for NTX-I structure determination coordinates of C alpha atoms of the NTX-I molecule were obtained and the position of NTX-I in the unit cell was derived.

摘要

通过气相扩散和透析法培养出了中亚眼镜蛇（Naja naja oxiana）毒液中神经毒素-I（NTX-I）的晶体。这些晶体属于空间群P2(1)2(1)2，晶胞参数为a = 25.19 Å，b = 75.59 Å，c = 36.09 Å，衍射分辨率达1.9 Å。不对称单元包含一个分子（Vm = 2.2 Å/Da）。以α-眼镜蛇毒素（CTX）分子作为NTX-I结构测定的起始模型，获得了NTX-I分子Cα原子的坐标，并推导出了NTX-I在晶胞中的位置。