来自中亚眼镜蛇毒液的神经毒素-1在1.9埃分辨率下的三维结构。

Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 A resolution.

作者信息

Nickitenko A V, Michailov A M, Betzel C, Wilson K S

机构信息

Institute of Crystallography of Russian Academy of Sciences, Moscow, Russian Federation.

出版信息

FEBS Lett. 1993 Apr 5;320(2):111-7. doi: 10.1016/0014-5793(93)80073-4.

DOI:10.1016/0014-5793(93)80073-4

PMID:8458425

Abstract

Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 A, b = 75.6 A, c = 35.9 A, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 A have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 A resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.

摘要

来自中亚眼镜蛇毒液的神经毒素-1（NTX-1）已通过坐滴气相扩散法结晶。晶体的晶胞参数为a = 25.2埃，b = 75.6埃，c = 35.9埃，属于空间群P2(1)2(1)2(1)。已使用Syntex P2(1)自动衍射仪记录了分辨率为1.9埃的三维数据。该毒素的原子结构已通过以α-眼镜蛇毒素（α-CTX）为搜索模型的分子置换法确定。已确定了534个非氢蛋白质原子的位置。该模型包含65个水分子。在1.9埃分辨率下精修得到的R因子为19.3%。已对NTX-1的二级和三级结构进行了分析，并与α-CTX的结构进行了比较。