从粉红平菇（Pleurotus djamor）中分离并鉴定一种新型核糖核酸酶

Isolation and characterization of a novel ribonuclease from the pink oyster mushroom Pleurotus djamor.

作者信息

Wu Xiangli, Zheng Suyue, Cui Li, Wang Hexiang, Ng Tzi Bun

机构信息

State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.

出版信息

J Gen Appl Microbiol. 2010 Jun;56(3):231-9. doi: 10.2323/jgam.56.231.

DOI:10.2323/jgam.56.231

PMID:20647680

Abstract

A 15-kDa RNase was purified from Pleurotus djamor using ion exchange chromatography and gel filtration. Its N-terminal amino acid sequence was different from previously reported RNase sequences of mushrooms belonging to the genus of Pleurotus and other genera. The RNase exhibited maximal RNase activity at pH 4.6 and 60 degrees C. Its activity toward polyhomoribonucleotides was poly(U) > poly(C) > poly(A) > poly(G). It inhibited proliferation of hepatoma cells and breast cancer cells. The ranking of inhibitory potencies of metal ions on RNase activity was Fe(3+)> Al(3+)> Ca(2+)> Hg(2+). The isolated RNase had a distinctive N-terminal sequence and optimum pH. It exhibited antiproliferative activity on tumor cells.

摘要

采用离子交换色谱法和凝胶过滤法从红平菇中纯化出一种15 kDa的核糖核酸酶。其N端氨基酸序列与先前报道的平菇属及其他属蘑菇的核糖核酸酶序列不同。该核糖核酸酶在pH 4.6和60℃时表现出最大核糖核酸酶活性。其对多聚同型核糖核苷酸的活性为聚尿苷酸＞聚胞苷酸＞聚腺苷酸＞聚鸟苷酸。它抑制肝癌细胞和乳腺癌细胞的增殖。金属离子对核糖核酸酶活性的抑制效力排序为Fe(3+)＞Al(3+)＞Ca(2+)＞Hg(2+)。分离出的核糖核酸酶具有独特的N端序列和最适pH。它对肿瘤细胞表现出抗增殖活性。