School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH), Stockholm, Sweden.
Proteomics. 2010 Nov;10(22):3984-96. doi: 10.1002/pmic.201000125.
An important part for understanding the complex machinery of living cells is to know the spatial distribution of proteins all the way from organ to organelle levels. An equally important part of proteomics is to map the subcellular distribution of all human proteins. Here, we discuss methodologies for systematic subcellular profiling with emphasis on the antibody-based approach performed as a part of the Human Protein Atlas project. The considerations made when creating the subcellular protein atlas and critical parameters of this approach are discussed.
了解活细胞复杂机制的一个重要部分是要知道蛋白质在器官到细胞器水平的空间分布。蛋白质组学的一个同样重要的部分是绘制所有人类蛋白质的亚细胞分布图谱。在这里，我们讨论了系统的亚细胞分析方法，重点是作为人类蛋白质图谱项目一部分的基于抗体的方法。本文讨论了在创建亚细胞蛋白质图谱时需要考虑的因素以及该方法的关键参数。
