Persson Anja, Hober Sophia, Uhlén Mathias
Royal Institute of Technology, School of Biotechnology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
Curr Opin Mol Ther. 2006 Jun;8(3):185-90.
The Human Protein Atlas is a comprehensive database that provides the protein expression profiles for a large number of human proteins, presented as immunohistological images from most human tissues. This review provides an overview of the contents of the atlas, discusses the project strategy and highlights the importance of open access for data validation and quality. Essential procedures that are implemented during antibody production and image generation, such as the use of protein epitope signature tags (PrEST) antigens, monospecific antibodies, tissue microarrays and thorough quality validation, are also discussed. The Human Protein Atlas is related to four other expression atlas initiatives, including, in particular, an upcoming protein atlas developed by the Sanger Institute.
人类蛋白质图谱是一个综合数据库，它提供了大量人类蛋白质的蛋白质表达谱，并以来自大多数人体组织的免疫组织学图像呈现。本综述概述了图谱的内容，讨论了项目策略，并强调了开放获取对于数据验证和质量的重要性。还讨论了在抗体生产和图像生成过程中实施的基本程序，例如使用蛋白质表位签名标签（PrEST）抗原、单特异性抗体、组织微阵列和全面的质量验证。人类蛋白质图谱与其他四项表达图谱计划相关，特别是与即将由桑格研究所开发的蛋白质图谱相关。
