Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Ann N Y Acad Sci. 2010 Jul;1201:137-46. doi: 10.1111/j.1749-6632.2010.05621.x.
Reprogramming of energy metabolism is one of the hallmarks of cancer. In normal conditions, cells rely on mitochondrial oxidative phosphorylation to provide energy for cellular activities. Cancer cells are characterized by increased glycolysis and reduced mitochondrial respiratory function. In the past decade, somatic mitochondrial DNA alterations are found to be common in all types of cancers. However, the functional significance of the altered cancer mitochondria is largely unknown. This is because the bulk of cancer properties are regulated by nuclear encoded genes. To overcome this problem, the transmitochondrial cybrid system, which allows the study of the effect of cancer mitochondria in a common nuclear background, has been used. Here we review the accumulating evidence that altered cancer mitochondria affect the respiratory chain function and oncogenic properties in vitro and in vivo using cybrid technologies.
能量代谢的重编程是癌症的特征之一。在正常情况下，细胞依赖于线粒体氧化磷酸化来为细胞活动提供能量。癌细胞的特点是糖酵解增加和线粒体呼吸功能降低。在过去的十年中，人们发现体细胞线粒体 DNA 改变在所有类型的癌症中都很常见。然而，改变的癌细胞线粒体的功能意义在很大程度上是未知的。这是因为大部分癌症特性是由核编码基因调控的。为了克服这个问题，人们使用了传递线粒体杂种细胞系统，该系统允许在共同的核背景下研究癌细胞线粒体的影响。在这里，我们综述了使用杂种细胞技术的体外和体内实验积累的证据，这些证据表明改变的癌细胞线粒体影响呼吸链功能和致癌特性。
