Department of Electrical and Computer Engineering, Texas A&M University, Zachry Engineering Center, College Station, TX 77843, USA.
Biosens Bioelectron. 2010 Sep 15;26(1):289-94. doi: 10.1016/j.bios.2010.06.034. Epub 2010 Jul 1.
We have developed a filter-chip and optical detection system for rapid antibiotic efficacy screening. The filter-chip consisted of a 1-mL reservoir and an anodic aluminum oxide (AAO) nanoporous membrane. Sample solution with liquid growth media, bacteria, and antibiotics was incubated in the reservoir for a specific period of time. The number of live bacteria on the surface of membrane was counted after the incubation with antibiotics and filtration. Using this biosensing system, we have demonstrated a 1-h antibiotic screening for patients' clinical samples, significantly faster than the conventional antibiotic susceptibility tests that typically take more than 24h. This rapid screening nature makes the filter-chip and detection system ideal for tailoring antibiotic treatment to individual patients by reducing the microbial antibiotic resistance, and improving the survival rate for patients suffering from postoperative infections.
我们开发了一种用于快速抗生素疗效筛选的滤片芯片和光学检测系统。该滤片芯片由 1 毫升的储液器和阳极氧化铝（AAO）纳米多孔膜组成。含有液体生长培养基、细菌和抗生素的样品溶液在储液器中孵育一段时间。孵育后，用抗生素过滤并计数膜表面的活菌数。使用这个生物传感系统，我们已经证明可以对患者的临床样本进行 1 小时的抗生素筛选，这比通常需要 24 小时以上的传统抗生素药敏试验快得多。这种快速筛选的特性使得滤片芯片和检测系统非常适合通过减少微生物的抗生素耐药性并提高术后感染患者的存活率来为个体患者定制抗生素治疗方案。
