Wang Feng, Wang Liu-xing, He Wei, Zhu Li-nan, Zhao Pei-rong, Fan Qing-xia
Department of Oncology, First Affiliated Hospital, Tumor Center, Zhengzhou University, Zhengzhou 450052, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jul;30(7):1552-7.
To explore the expression of stathmin gene in esophageal squamous cell carcinoma (ESCC) and its correlation to oncogenesis of ESCC.
Three ESCC cell lines, 75 ESCC samples, 25 tumor-adjacent samples and 30 normal esophageal mucosa samples were examined for the expression of stathmin mRNA and protein by in situ hybridization and immunohistochemistry, respectively. The correlations of stathmin expression to the clinicopathological features of the patients were analyzed.
Overexpression of stathmin mRNA and protein was found in 3 ESCC cell lines EC9706, Eca109 and EC-1, with the positive expression rates exceeding 80%. The positive rates of stathmin mRNA and protein in ESCC samples were 82.7% and 81.3%, respectively. There were significant differences in the relative contents of stathmin mRNA and protein among normal mucosa tissue, tumor-adjacent tissue and cancer tissue (chi2=19.204 and 25.03, respectively, P<0.01). In addition, a positive correlation was noted between stathmin mRNA and protein expressions in ESCC (r=0.413, P=0.000). The relative contents of stathmin mRNA and protein were significantly correlated to the differentiation degree, lymph node metastasis, invasive depth and TNM stage of ESCC (P<0.05).
The expression of stathmin mRNA and protein is upregulated in ESCC with correlation to the differentiation degree, lymph node metastasis, invasive depth and TNM stage of ESCC, suggesting the possible involvement of stathmin in the oncogenesis of ESCC. Combined detection of stathmin mRNA and protein may prove valuable for early diagnosis and prognosis of ESCC, and stathmin may serve as a potential molecular target for biotherapy of the tumor.
探讨Stathmin基因在食管鳞状细胞癌（ESCC）中的表达及其与ESCC发生的相关性。
分别采用原位杂交和免疫组织化学方法检测3种ESCC细胞系、75例ESCC标本、25例癌旁标本及30例正常食管黏膜标本中Stathmin mRNA和蛋白的表达情况。分析Stathmin表达与患者临床病理特征的相关性。
在3种ESCC细胞系EC9706、Eca109和EC-1中发现Stathmin mRNA和蛋白过表达，阳性表达率均超过80%。ESCC标本中Stathmin mRNA和蛋白的阳性率分别为82.7%和81.3%。正常黏膜组织、癌旁组织和癌组织中Stathmin mRNA和蛋白的相对含量差异有统计学意义（分别为χ2=19.204和25.03，P<0.01）。此外，ESCC中Stathmin mRNA与蛋白表达呈正相关（r=0.413，P=0.000）。Stathmin mRNA和蛋白的相对含量与ESCC的分化程度、淋巴结转移、浸润深度及TNM分期显著相关（P<0.05）。
ESCC中Stathmin mRNA和蛋白表达上调，与ESCC的分化程度、淋巴结转移、浸润深度及TNM分期相关，提示Stathmin可能参与ESCC的发生。联合检测Stathmin mRNA和蛋白可能对ESCC的早期诊断和预后评估有重要价值，且Stathmin可能成为肿瘤生物治疗的潜在分子靶点。
