Liu Sha, Xiong Feng, Liu En-mei, Zhu Min, Lei Pei-yun
Department of Endocrinology, Children's Hospital Affiliated to Chongqing Medical University, Chongqing 400014, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jul;30(7):1573-6.
To observe the effect of 1,25(OH)2D3 on thyroid inflammation and Th1/Th2 cells in rats with experimental autoimmune thyroiditis (EAT).
Forty-eight female Wistar rats were randomly divided into 4 groups, namely the prevention group treated with 1, 25-(OH)2D3 from 0 to the 6th week (n=10), treatment group with 1,25(OH)2D3 treatment from the 2nd to the 8th week (n=10) after immune sensitization, positive control group (n=12) and the negative control group (n=16). All the rats were challenged with porcine thyroglobulin for immune sensitization until the 6th or 8th week except for those in the negative control group. In the prevention group and treatment group, the rats received 1,25(OH)2D3 at 5 microg/kg by intraperitoneal injection every other day, while those in the positive and negative control groups were given peanut oil instead. The thyroid pathologies, serum autoantibody level and cytokine levels were examined after the treatments.
The thyroid gland remained structurally intact in the negative control group. In the positive control group, the thyroid showed obvious inflammatory change with structural disruption and even disappearance of the thyroid follicle. The structure of the thyroid gland follicles was intact in the prevention group and treatment group. No significant differences were found in the autoantibody and cytokine levels between the prevention group and negative control group (P>0.05). Compared with the positive control groups, the autoantibody and IFN-gamma and IL-12 levels decreased significantly in the treatment group, but the levels of IL-4 and IL-10 were markedly increased (P<0.05).
1,25(OH)2D3 given before the establishment of the EAT model helps maintain structural integrity of the thyroid gland and normal levels of the antibodies and cytokines in rats. 1,25(OH)2D3 can ameliorate the pathological changes of the thyroid gland and correct the cytokine disequilibrium in rats with EAT.
观察1,25(OH)₂D₃对实验性自身免疫性甲状腺炎（EAT）大鼠甲状腺炎症及Th1/Th2细胞的影响。
48只雌性Wistar大鼠随机分为4组，即0至6周用1,25-(OH)₂D₃治疗的预防组（n = 10）、免疫致敏后第2至8周用1,25(OH)₂D₃治疗的治疗组（n = 10）、阳性对照组（n = 12）和阴性对照组（n = 16）。除阴性对照组外，所有大鼠均用猪甲状腺球蛋白进行免疫致敏至第6周或第8周。预防组和治疗组大鼠每隔一天腹腔注射5μg/kg的1,25(OH)₂D₃，而阳性和阴性对照组大鼠则给予花生油。治疗后检测甲状腺病理学、血清自身抗体水平和细胞因子水平。
阴性对照组甲状腺结构保持完整。阳性对照组甲状腺出现明显炎症改变，结构破坏，甲状腺滤泡甚至消失。预防组和治疗组甲状腺滤泡结构完整。预防组与阴性对照组自身抗体和细胞因子水平比较，差异无统计学意义（P > 0.05）。与阳性对照组相比，治疗组自身抗体、IFN-γ和IL-12水平显著降低，但IL-4和IL-10水平显著升高（P < 0.05）。
在EAT模型建立前给予1,25(OH)₂D₃有助于维持大鼠甲状腺结构完整性及抗体和细胞因子的正常水平。1,25(OH)₂D₃可改善EAT大鼠甲状腺的病理变化并纠正细胞因子失衡。
