Department of Cardiology, Puai Hospital, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
Am J Pathol. 2010 Sep;177(3):1122-30. doi: 10.2353/ajpath.2010.090889. Epub 2010 Jul 22.
To establish a model of coronary thrombotic microembolism in rats, either automicrothrombotic particulates (CM group) or saline control (SHAM group) was injected into temporarily clamped aortas of male Sprague-Dawley rats. After automicrothrombotic particulate injection, serum c-troponin I and von Willebrand factor levels, the no-flow area as evaluated by Thioflavin S, myocardial leukocyte infiltration levels, myocardial expressions of tumor necrosis factor alpha and interleukin-6, the percentage of arterioles obstructed by thrombosis, and myocardial fibrosis were all significantly increased whereas cardiac function as evaluated by echocardiography and hemodynamic measurements were significantly reduced compared with the sham group. Thus, aortic automicrothrombotic particulate injection could induce coronary microembolism in rats, and this model could be of value in improving the understanding of pathophysiology of coronary microembolism.
为了建立大鼠冠状动脉血栓性微栓塞模型，将自动微血栓颗粒（CM 组）或生理盐水对照（SHAM 组）注入雄性 Sprague-Dawley 大鼠临时夹闭的主动脉中。在自动微血栓颗粒注射后，血清 c-肌钙蛋白 I 和血管性血友病因子水平、噻唑蓝 S 评估的无血流区、心肌白细胞浸润水平、心肌肿瘤坏死因子-α和白细胞介素-6 的表达、被血栓阻塞的小动脉百分比以及心肌纤维化均显著增加，而超声心动图和血流动力学测量评估的心脏功能则显著降低与假手术组相比。因此，主动脉自动微血栓颗粒注射可在大鼠中诱导冠状动脉微栓塞，该模型可能有助于改善对冠状动脉微栓塞病理生理学的理解。
