McCleary V L, Akers T K, Aasen G H
Dept. of Phys., UND School of Medicine, Grand Forks 58202.
Biomed Sci Instrum. 1991;27:205-17.
Pulsed electromagnetic fields [EMF] and electric fields have been demonstrated to promote osteogenesis and wound healing. Pulsed EMF's have been approved since 1979 by the FDA, and are highly effective in the treatment of non-union fractures. Increased linear growth, cellular proliferation, cAMP and uptake of tritiated thymidine have been documented on short term exposure. Yet the mechanisms and the changes that occur have been difficult to quantify. Fluorescence, light, and electron microscopy were utilized in this study to assess any histological changes in bone. During incubation chick embryos were exposed to magnets oriented in various positions. Controls were oriented similarly using galvanized steel plugs. Field density in the center of the field was measure by a gaussmeter with a transverse probe. Each chick embryo in its magnetic field was isolated from the magnetic fields of others by being encased in a steel box. Intramembranous [calvaria] and endochondral [tibia] ossification were studied. Fluorescent dyes were micropipetted intravascularly at various stages of chick development. The tissues were fixed in methacrylate and stained for histomorphological study.
脉冲电磁场（EMF）和电场已被证明可促进成骨作用和伤口愈合。自1979年以来，脉冲电磁场已获得美国食品药品监督管理局（FDA）的批准，并且在治疗骨不连骨折方面非常有效。短期暴露后，已记录到线性生长增加、细胞增殖、环磷酸腺苷（cAMP）以及氚标记胸腺嘧啶核苷摄取增加。然而，其发生的机制和变化难以量化。本研究利用荧光显微镜、光学显微镜和电子显微镜来评估骨骼的任何组织学变化。在孵化期间，将鸡胚暴露于不同位置的磁体。对照组使用镀锌钢塞进行类似的定位。用场强仪的横向探头测量磁场中心的场密度。每个置于磁场中的鸡胚都被装在一个钢箱中，以与其他鸡胚的磁场隔离开来。研究了膜内（颅骨）和软骨内（胫骨）骨化。在鸡发育的不同阶段，通过微量移液器将荧光染料注入血管内。将组织固定在甲基丙烯酸酯中，并进行染色以进行组织形态学研究。
