Institut für Technische Chemie, Leibniz Universität Hannover, Callinstr. 3, 30167, Hannover, Germany.
Appl Microbiol Biotechnol. 2010 Sep;88(1):11-22. doi: 10.1007/s00253-010-2743-8. Epub 2010 Jul 22.
One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique to analyze a wide variety of organic compounds. Thus, infrared sensors are ideal instruments for bioprocess monitoring. The sensors are non-invasive, have no time delay due to sensor response times, and have no influence on the bioprocess itself. No sampling is necessary, and several components can be analyzed simultaneously. In general, the direct monitoring of substrates, products, metabolites, as well as the biomass itself is possible. In this review article, insights are provided into the different applications of infrared spectroscopy for bioprocess monitoring and the complex data interpretation. Different analytical techniques are presented as well as example applications in different areas.
生物过程工程的主要目标之一是实时监测重要的过程变量。这是精确过程控制的基础，对于提高生产力以及准确记录整个生产过程至关重要。红外光谱是分析各种有机化合物的强大分析技术。因此，红外传感器是生物过程监测的理想仪器。这些传感器是非侵入式的，由于传感器响应时间而没有时间延迟，并且对生物过程本身没有影响。不需要采样，并且可以同时分析多个组件。通常，可以直接监测底物、产物、代谢物以及生物质本身。在这篇综述文章中，我们深入了解了红外光谱在生物过程监测中的不同应用以及复杂的数据解释。还介绍了不同的分析技术以及在不同领域的应用实例。
