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使用近红外(NIR)光密度传感器对大规模微藻培养进行实时监测和自动密度控制。

Real-time monitoring and automatic density control of large-scale microalgal cultures using near infrared (NIR) optical density sensors.

作者信息

Sandnes J M, Ringstad T, Wenner D, Heyerdahl P H, Källqvist T, Gislerød H R

机构信息

Norwegian University of Life Sciences (UMB), Department of Plant and Environmental Sciences, P.B. 5003, 1432 As, Norway.

出版信息

J Biotechnol. 2006 Mar 23;122(2):209-15. doi: 10.1016/j.jbiotec.2005.08.034. Epub 2005 Oct 25.

DOI:10.1016/j.jbiotec.2005.08.034
PMID:16253371
Abstract

Signals from near infrared (NIR) light transmittance sensors were used for both real-time monitoring of algal biomass density in growing mass cultures (200l tubular biofences), and also as feedback in a system that controlled the density of the culture by automatic injection of fresh growth medium. When operated in a semi-continuous production mode between predefined density values, diurnal growth patterns were recorded on-line that provided information on the dynamics of the microalgal cultures with respect to environmental conditions. The bioreactor system was also programmed to operate in constant biomass density mode, thereby maintaining the culture at the optimal population density (OPD), and sustaining high biomass production levels. The system has potential for operating a dynamic density set point for microalgal cultures where the optimal population density varies as a function of ambient growing conditions.

摘要

来自近红外(NIR)光透射传感器的信号用于实时监测大规模培养(200升管状生物围栏)中的藻类生物量密度,同时也作为一个系统的反馈,该系统通过自动注入新鲜生长培养基来控制培养物的密度。当在预定义密度值之间以半连续生产模式运行时,在线记录了昼夜生长模式,这些模式提供了有关微藻培养物相对于环境条件的动态信息。生物反应器系统还被编程为以恒定生物量密度模式运行,从而将培养物维持在最佳种群密度(OPD),并维持高生物量生产水平。该系统有潜力为微藻培养物运行动态密度设定点,其中最佳种群密度随环境生长条件而变化。

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