Department of Bionano System Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
J Mater Sci Mater Med. 2010 Sep;21(9):2551-9. doi: 10.1007/s10856-010-4102-9. Epub 2010 Jul 22.
In this study, a good combination consisting of electrospun titanium dioxide (TiO(2)) nanofibers incorporated with high purity hydroxyapatite (HAp) nanoparticles (NPs) and antimicrobial silver NPs is introduced for hard tissue engineering applications. The synthesized nanofibers were characterized by various state of art techniques like; SEM, XRD, TEM, TEM EDS and XPS analyses. SEM results confirmed well oriented nanofibers and good dispersion of HAp and silver NPs, respectively. XRD results demonstrated well crystalline feature of three components used for electrospinning. Silver NPs were having a diameter in range of 5-8 nm indicated by TEM analysis. Moreover, TEM EDS analysis demonstrated the presence of each component with good dispersion over TiO(2) nanofiber. The surface analyses of nanofibers were investigated by XPS which indicated the presence of silver NPs on the surfaces of nanofibers. The obtained nanofibers were checked for antimicrobial activity by using two model organisms E. coli and S. aureus. Subsequently, antimicrobial tests have indicated that the prepared nanofibers do possess high bactericidal effect. Accordingly, these results strongly recommend the use of obtained nanofiber mats as future implant materials.
在这项研究中，引入了一种由电纺二氧化钛（TiO2）纳米纤维与高纯度羟基磷灰石（HAp）纳米颗粒（NPs）和抗菌银 NPs 良好结合的组合，用于硬组织工程应用。合成的纳米纤维通过各种先进技术进行了表征，如 SEM、XRD、TEM、TEM EDS 和 XPS 分析。SEM 结果证实了纳米纤维具有良好的取向性，以及 HAp 和银 NPs 的良好分散性。XRD 结果表明，用于电纺丝的三种成分具有良好的结晶特性。TEM 分析表明，银 NPs 的直径在 5-8nm 范围内。此外，TEM EDS 分析表明，各成分在 TiO2 纳米纤维上具有良好的分散性。通过使用两种模型生物大肠杆菌和金黄色葡萄球菌对纳米纤维的表面分析进行了研究。随后，抗菌试验表明，所制备的纳米纤维确实具有很高的杀菌效果。因此，这些结果强烈推荐使用所得到的纳米纤维垫作为未来的植入材料。
