College of Chemistry, Chongqing Normal University, Chongqing 400047, China.
Z Naturforsch C J Biosci. 2010 May-Jun;65(5-6):387-90. doi: 10.1515/znc-2010-5-611.
Extraction of mannan oligosaccharides from the yeast cell wall and methods of deproteinization were studied. We extracted crude mannan oligosaccharides by the dilute alkali-Sevage method. The percentages of deproteinization and mannan oligosaccharide loss were compared as indexes using the Sevage method, trichloroacetic acid method, and hydrochloric acid method. The results showed that the hydrochloric acid method exhibited the highest percentage of deproteinization, but only a little higher percentage of mannan oligosaccharide loss than the other two methods.
研究了从酵母细胞壁中提取甘露寡糖和脱蛋白的方法。我们采用稀碱-Sevage 法提取粗甘露寡糖。以 Sevage 法、三氯乙酸法和盐酸法为比较指标，比较了脱蛋白率和甘露寡糖损失率。结果表明，盐酸法的脱蛋白率最高，但与其他两种方法相比，甘露寡糖损失率仅略高。
