Science and Research Branch, Islamic Azad University, Tehran, Iran.
Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Iran Biomed J. 2023 Sep 1;27(5):320-25. doi: 10.61186/ibj.27.5.320. Epub 2023 Feb 12.
Mannoproteins, mannose-glycosylated proteins, play an important role in biological processes and have various applications in industries. Several methods have been already used for the extraction of mannoproteins from yeast cell-wall. The aim of this study was to evaluate the extraction and deproteinization of mannan oligosaccharide from the Kluyveromyces (K.) marxianus mannoprotein.
To acquire crude mannan oligosaccharides, K. marxianus mannoproteins were deproteinized by the Sevage, trichloroacetic acid, and hydrochloric acid (HCL) methods. Total nitrogen, crude protein content, fat, carbohydrate and ash content were measured according to the monograph prepared by the meeting of the Joint FAO/WHO Expert Committee and standard. Mannan oligosaccharide loss, percentage of deproteinization, and chemical composition of the product were assessed to check the proficiency of different methods.
Highly purified (95.4%) mannan oligosaccharide with the highest deproteinization (97.33 ± 0.4%) and mannan oligosaccharide loss (25.1 ± 0.6%) were obtained following HCl method.
HCl, was the most appropriate deproteinization method for the removal of impurities. This preliminary data will support future studies to design scale-up procedures.
甘露糖蛋白，即糖基化的甘露糖蛋白，在生物过程中发挥着重要作用，并在工业中有多种应用。已经有几种方法用于从酵母细胞壁中提取甘露糖蛋白。本研究旨在评估从克鲁维酵母（K.）甘露糖蛋白中提取和脱蛋白甘露聚糖寡糖的方法。
为了获得粗制的甘露聚糖寡糖，采用 Sevage、三氯乙酸和盐酸（HCL）法对克鲁维酵母甘露糖蛋白进行脱蛋白处理。根据粮农组织/世界卫生组织联合专家委员会会议和标准编写的专论，测定总氮、粗蛋白含量、脂肪、碳水化合物和灰分。评估甘露聚糖寡糖损失、脱蛋白率和产品的化学成分，以检查不同方法的效果。
采用 HCl 法可获得高度纯化（95.4%）的甘露聚糖寡糖，脱蛋白率最高（97.33±0.4%），甘露聚糖寡糖损失率最低（25.1±0.6%）。
HCl 是去除杂质的最适宜脱蛋白方法。这些初步数据将为未来的设计放大程序提供支持。
