Velarde G, Ait-Aissa S, Gillet C, Rogerieux F, Lambre C, Vindimian E, Porcher J M
Laboratoire de Biochimie et Toxicologie in vitro, Institut National de l'Environnement Industriel et des Risques (INERIS), F-60550 Verneuil-en-Halatte, France.
Toxicol In Vitro. 1999 Aug-Oct;13(4-5):723-7. doi: 10.1016/s0887-2333(99)00048-x.
The toxicity of pentachlorophenol (PCP), a polluting substance believed to exert a narcotic effect, was assayed using the Caco-2 cell line as a model. In order to assess this toxicity as fully as possible, several viability tests, each examining different endpoints, have been used. Neutral red uptake was found to be more sensitive to PCP than MTT and Alamar Blue tests. Transepithelial electrical resistance (TEER) was shown to be the most sensitive to PCP at concentrations and exposure times where the Alamar Blue, LDH leakage and Blue Dextran passage did not evidence any effect. Blue Dextran passage and optical microscopy revealed cellular detachment at concentrations where LDH and Alamar Blue showed little or no cytotoxicity. Thus, PCP seems to affect the integrity of the intestinal barrier at levels where no cytotoxicity is seen. Our results support the notion that TEER can be used as a very sensitive method for evaluating membrane-perturbing toxicants.
五氯苯酚（PCP）是一种被认为具有麻醉作用的污染物质，其毒性以Caco-2细胞系为模型进行了测定。为了尽可能全面地评估这种毒性，使用了几种活力测试，每种测试都检查不同的终点。发现中性红摄取对PCP比MTT和Alamar Blue测试更敏感。在Alamar Blue、乳酸脱氢酶（LDH）泄漏和蓝色葡聚糖通透未显示任何影响的浓度和暴露时间下，跨上皮电阻（TEER）对PCP最为敏感。蓝色葡聚糖通透和光学显微镜检查显示，在LDH和Alamar Blue显示很少或没有细胞毒性的浓度下，细胞发生脱离。因此，PCP似乎在未见细胞毒性的水平上影响肠道屏障的完整性。我们的结果支持这样一种观点，即TEER可作为评估膜干扰性毒物的一种非常敏感的方法。
