Horváth Lenke, Umehara Yuki, Jud Corinne, Blank Fabian, Petri-Fink Alke, Rothen-Rutishauser Barbara
Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg, Switzerland.
Department of Respiratory Medicine, Bern University Hospital, CH-3010 Bern, Switzerland.
Sci Rep. 2015 Jan 22;5:7974. doi: 10.1038/srep07974.
Intensive efforts in recent years to develop and commercialize in vitro alternatives in the field of risk assessment have yielded new promising two- and three dimensional (3D) cell culture models. Nevertheless, a realistic 3D in vitro alveolar model is not available yet. Here we report on the biofabrication of the human air-blood tissue barrier analogue composed of an endothelial cell, basement membrane and epithelial cell layer by using a bioprinting technology. In contrary to the manual method, we demonstrate that this technique enables automatized and reproducible creation of thinner and more homogeneous cell layers, which is required for an optimal air-blood tissue barrier. This bioprinting platform will offer an excellent tool to engineer an advanced 3D lung model for high-throughput screening for safety assessment and drug efficacy testing.
近年来,在风险评估领域大力开展体外替代物的研发和商业化工作,已产生了新的、有前景的二维和三维(3D)细胞培养模型。然而,目前仍没有逼真的3D体外肺泡模型。在此,我们报告利用生物打印技术生物制造由内皮细胞、基底膜和上皮细胞层组成的人气血组织屏障类似物。与手工方法不同,我们证明该技术能够自动且可重复地创建更薄且更均匀的细胞层,这是优化气血组织屏障所必需的。这个生物打印平台将为构建用于安全评估和药物疗效测试的高通量筛选的先进3D肺模型提供一个出色的工具。