Kempf & Pfaltz Histological Diagnostics, Research Unit, Zurich, Switzerland.
Hum Pathol. 2010 Oct;41(10):1369-79. doi: 10.1016/j.humpath.2010.01.023. Epub 2010 Jul 22.
Polyomaviruses have been linked to diseases of immunosuppressed patients. We sought to determine the prevalence of Merkel cell polyomavirus in benign epithelial skin neoplasms and nonmelanoma skin cancer of immunosuppressed renal transplant recipients and long-term dialysis patients. Merkel cell polyomavirus DNA was detected by polymerase chain reaction (PCR) in 2 (10%) of 20 patients, in carcinomas in situ (Bowen's disease). In one of our patients with Merkel cell polyomavirus-positive carcinoma in situ, 9 (39.1%) of 23 skin lesions at various anatomical locations tested positive for Merkel cell polyomavirus sequences by PCR, including all of his common warts (4/4), half of his carcinoma in situ lesions (3/6), and 2 of his 3 seborrheic keratoses. In a second cohort of immunosuppressed renal transplant recipients, Merkel cell polyomavirus DNA was found in 1 (6.3%) of 16 common warts and in 2 (9.5%) of 21 carcinomas in situ. In immunocompetent individuals, Merkel cell polyomavirus DNA was found in 2 (6.7%) of 30 common warts and in 2 (8.3%) of 24 carcinomas in situ. DNA of other human polyomaviruses was not detected in any of the investigated skin neoplasms. We conclude that common warts and carcinomas in situ can be positive for Merkel cell polyomavirus in immunosuppressed as well as immunocompetent individuals. Remarkably, some of the Merkel cell polyomavirus-positive common warts did not contain human papillomavirus. Furthermore, Merkel cell polyomavirus can be found in various skin neoplasms of the same individual.
多瘤病毒与免疫抑制患者的疾病有关。我们试图确定在免疫抑制的肾移植受者和长期透析患者的良性上皮皮肤肿瘤和非黑素瘤皮肤癌中 Merkel 细胞多瘤病毒的流行率。通过聚合酶链反应（PCR）检测到 20 例患者中的 2 例（10％）存在 Merkel 细胞多瘤病毒 DNA，这些患者存在原位癌（鲍文氏病）。在我们的一位 Merkel 细胞多瘤病毒阳性原位癌患者中，在各种解剖位置的 23 个皮肤病变中，有 9 个（39.1％）通过 PCR 检测到 Merkel 细胞多瘤病毒序列呈阳性，包括他所有的普通疣（4/4），他的原位癌病变的一半（3/6），以及他的 3 个脂溢性角化病中的 2 个。在第二组免疫抑制的肾移植受者中，在 16 例普通疣中有 1 例（6.3％）和在 21 例原位癌中有 2 例（9.5％）发现 Merkel 细胞多瘤病毒 DNA。在免疫功能正常的个体中，在 30 例普通疣中有 2 例（6.7％）和在 24 例原位癌中有 2 例（8.3％）发现 Merkel 细胞多瘤病毒 DNA。在任何研究的皮肤肿瘤中均未检测到其他人类多瘤病毒的 DNA。我们的结论是，在免疫抑制和免疫功能正常的个体中，普通疣和原位癌可能会呈 Merkel 细胞多瘤病毒阳性。值得注意的是，一些 Merkel 细胞多瘤病毒阳性的普通疣中不包含人乳头瘤病毒。此外，Merkel 细胞多瘤病毒可在同一个体的各种皮肤肿瘤中找到。
