Shamanin V, Glover M, Rausch C, Proby C, Leigh I M, zur Hausen H, de Villiers E M
Division for Tumorvirus Characterization, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Cancer Res. 1994 Sep 1;54(17):4610-3.
A total of 118 biopsies from skin lesions of 46 renal allograft patients was analyzed for human papillomavirus (HPV) DNA by polymerase chain reaction with degenerate primers and also partially by subsequent sequencing of the amplified fragment. Sixty-two % of the benign proliferations (31 of 50) contained DNA of known HPV types as well as HPV sequences related to a number of epidermodysplasia verruciformis-associated HPV types. HPV DNA sequences were found in 14 (56%) of 25 biopsies from squamous cell and basal cell carcinomas. One squamous cell carcinoma contained HPV 41 DNA. A novel 640-base pair fragment sharing homology with HPV 29 (82.7%) was found in 15% (3 of 20) of squamous cell carcinomas, in 9.4% (3 of 32) of dysplastic warts and in 8.5% (4 of 47) common warts. The remaining positive carcinoma biopsies contained HPV-related DNA in such a low copy number that additional analysis is required. The identification of new HPV types in skin cancers of immunosuppressed patients (other than epidermodysplasia verruciformis patients) further expands the spectrum of HPV-linked human malignancies and permits new approaches to study the pathogenesis of skin cancers.
采用简并引物通过聚合酶链反应对46例肾移植患者皮肤病变的118份活检标本进行人乳头瘤病毒（HPV）DNA分析，并对扩增片段进行部分测序。62%的良性增生（50例中的31例）含有已知HPV类型的DNA以及与多种疣状表皮发育不良相关HPV类型有关的HPV序列。在25份鳞状细胞癌和基底细胞癌活检标本中，14份（56%）检测到HPV DNA序列。1例鳞状细胞癌含有HPV 41 DNA。在15%（20例中的3例）的鳞状细胞癌、9.4%（32例中的3例）的发育异常疣和8.5%（47例中的4例）的寻常疣中发现了一个与HPV 29具有同源性（82.7%）的640个碱基对的新片段。其余阳性癌活检标本中HPV相关DNA的拷贝数很低，需要进一步分析。在免疫抑制患者（疣状表皮发育不良患者除外）的皮肤癌中鉴定出新的HPV类型，进一步扩大了HPV相关人类恶性肿瘤的范围，并为研究皮肤癌的发病机制提供了新的途径。
