Department of Food, Agricultural and Biological Engineering, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
Bioresour Technol. 2010 Dec;101(23):9213-20. doi: 10.1016/j.biortech.2010.06.114. Epub 2010 Jul 22.
In this study, the effects of different wavelengths of light (UV, blue, green, yellow, red) and white light on lignin peroxidase (LiP), protein, biomass and exo-polysaccharide production and glucose uptake by Phanerochaete chrysosporium BKM-F-1767 were determined. The experiments were conducted under aerated (CS) and oxygenated (RS) culture conditions. The results showed that only green light significantly increased maximum LiP production (by 20% and 27% in CS and RS cultures respectively). Green light also increased biomass production in oxygenated cultures (RS). Blue and UV light both significantly reduced maximum LiP activity. Yellow, red and white lights had mixed effects on culture properties. This is the first time that the effects of different wavelengths of light on lignin peroxidase production and other culture properties have been investigated. The novel findings may be important in improving the yield of lignin modifying enzymes for biomass conversion processes and understanding their regulation.
本研究旨在确定不同波长的光（UV、蓝、绿、黄、红）和白光对木质素过氧化物酶（LiP）、蛋白质、生物量以及外多糖生产和葡萄糖摄取的影响，实验在通气（CS）和充氧（RS）培养条件下进行。结果表明，只有绿光显著提高了 LiP 的最大产量（分别在 CS 和 RS 培养中提高了 20%和 27%）。绿光还增加了充氧培养中的生物量（RS）。蓝、紫外光均显著降低了 LiP 的最大活性。黄、红、白光对培养特性的影响各有不同。这是首次研究不同波长的光对木质素过氧化物酶生产和其他培养特性的影响。这些新发现可能对提高木质素修饰酶用于生物质转化过程的产量以及理解其调控机制具有重要意义。
