呼吸道合胞病毒附着蛋白中心无糖基化区域的体液反应。
Humoral response to the central unglycosylated region of the respiratory syncytial virus attachment protein.
机构信息
Division of Infectious Diseases, Department of Medicine, University of Rochester School of Medicine and Dentistry and Infectious Diseases Unit, Rochester General Hospital, 601 Elmwood Avenue Box 689, Rochester, NY 14642, USA. Yoshihiko
出版信息
Vaccine. 2010 Aug 31;28(38):6242-6. doi: 10.1016/j.vaccine.2010.07.011. Epub 2010 Jul 23.
Abstract
To characterize the humoral response to the unglycosylated central region of the respiratory syncytial virus (RSV) attachment (G) protein, we generated glutathione S-transferase (GST)-RSV G subdomains (central core (CC), residues 151-190; proximal central core (PCC), 151-172; and distal central core (DCC), 173-190) to screen paired sera from RSV subtype A- or B-infected adults in hospitalized or outpatient settings. Following RSV infection, a >or=4-fold increase in homo- and heterosubtypic IgG response was noted in most subjects against the RSV G CC and PCC regions; in contrast, such titer increases against the RSV G DCC was only noted in a homosubtypic manner. Our results have implications for RSV G-based serological diagnostics and vaccine development.
摘要
为了描述呼吸道合胞病毒（RSV）附着（G）蛋白未糖基化的中心区域的体液免疫反应，我们生成了谷胱甘肽 S-转移酶（GST）-RSV G 亚结构域（中心核心（CC），残基 151-190；近端中心核心（PCC），151-172；和远端中心核心（DCC），173-190），以筛选住院或门诊环境中 RSV 亚型 A 或 B 感染成人的配对血清。在 RSV 感染后，大多数受试者对 RSV G CC 和 PCC 区域表现出同源和异源 IgG 反应的>或=4 倍增加；相比之下，仅以同型方式观察到针对 RSV G DCC 的这种滴度增加。我们的结果对基于 RSV G 的血清学诊断和疫苗开发具有重要意义。
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