聚亚乙基亚胺与 DNA 的结合热力学和 DNA 的凝聚。
Thermodynamics of polyethylenimine-DNA binding and DNA condensation.
机构信息
Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Alberta, Canada.
出版信息
Biophys J. 2010 Jul 7;99(1):201-7. doi: 10.1016/j.bpj.2010.04.016.
Abstract
In this study, polyethylenimine (PEI) binding to DNA was examined by isothermal titration calorimetry. Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the enthalpy change of this binding mode is positive, and PEI is deprotonated to bind to DNA. Another likely binding mode involves external binding of PEI to the DNA phosphate backbone, accompanied with DNA condensation. The enthalpy change is negative and PEI is protonated when it binds to DNA in this mode. The intrinsic enthalpy change of first binding mode is 1.1 kJ/mol and -0.88 kJ/mol for the second binding mode. This result implies that the PEI is rearranged from the groove to the phosphate backbone of DNA when DNA is condensed. The mechanism of DNA condensation caused by PEI is discussed in this study.
摘要
在这项研究中，通过等温热力学滴定法研究了聚乙烯亚胺 (PEI) 与 DNA 的结合。发现了两种结合模式来描述这些聚电解质在缓冲液和水中的相互作用。一种结合类型涉及 PEI 结合到 DNA 凹槽中，因为这种结合模式的焓变是正的，并且 PEI 去质子化以与 DNA 结合。另一种可能的结合模式涉及 PEI 外部结合到 DNA 磷酸骨架上，伴随着 DNA 凝聚。当 PEI 以这种模式与 DNA 结合时，焓变为负，PEI 质子化。第一种结合模式的内在焓变为 1.1 kJ/mol，第二种结合模式为-0.88 kJ/mol。该结果表明，当 DNA 凝聚时，PEI 从凹槽重新排列到 DNA 的磷酸骨架上。本文讨论了 PEI 引起 DNA 凝聚的机制。
相似文献
1
Thermodynamics of polyethylenimine-DNA binding and DNA condensation.聚亚乙基亚胺与 DNA 的结合热力学和 DNA 的凝聚。
Biophys J. 2010 Jul 7;99(1):201-7. doi: 10.1016/j.bpj.2010.04.016.
2
Low Molecular Weight Branched PEI Binding to Linear DNA.
Chem Pharm Bull (Tokyo). 2016;64(10):1484-1491. doi: 10.1248/cpb.c16-00454.
3
The energetics of HMG box interactions with DNA: thermodynamics of the DNA binding of the HMG box from mouse sox-5.HMG盒与DNA相互作用的能量学：小鼠sox-5的HMG盒与DNA结合的热力学
J Mol Biol. 1999 Dec 10;294(4):997-1013. doi: 10.1006/jmbi.1999.3285.
4
Thermodynamics of DNA condensation caused by mn2+ binding.
Chem Pharm Bull (Tokyo). 2008 Mar;56(3):247-9. doi: 10.1248/cpb.56.247.
5
Impact of molecular weight and degree of conjugation on the thermodynamics of DNA complexation and stability of polyethylenimine-graft-poly(ethylene glycol) copolymers.分子量和共轭度对聚乙烯亚胺接枝聚乙二醇共聚物的DNA络合热力学及稳定性的影响。
Biophys Chem. 2015 Aug-Sep;203-204:12-21. doi: 10.1016/j.bpc.2015.04.005. Epub 2015 May 7.
6
Thermodynamics of DNA binding and condensation: isothermal titration calorimetry and electrostatic mechanism.DNA结合与凝聚的热力学：等温滴定量热法与静电机制
J Mol Biol. 2000 Mar 3;296(4):1053-63. doi: 10.1006/jmbi.1999.3470.
7
Molecular dynamics simulations of PEI mediated DNA aggregation.PEI 介导的 DNA 聚集的分子动力学模拟。
Biomacromolecules. 2011 Oct 10;12(10):3698-707. doi: 10.1021/bm2009476. Epub 2011 Sep 22.
8
Thermodynamic analysis of binding and protonation in DOTAP/DOPE (1:1): DNA complexes using isothermal titration calorimetry.使用等温滴定量热法对DOTAP/DOPE（1:1）：DNA复合物中的结合和质子化进行热力学分析。
Biophys Chem. 2003 May 1;104(1):67-78. doi: 10.1016/s0301-4622(02)00339-3.
9
Binding of netropsin to several DNA constructs: evidence for at least two different 1:1 complexes formed from an -AATT-containing ds-DNA construct and a single minor groove binding ligand.纺锤菌素与几种DNA构建体的结合：由含-AATT的双链DNA构建体和单个小沟结合配体形成至少两种不同1:1复合物的证据。
Biophys Chem. 2007 Mar;126(1-3):186-96. doi: 10.1016/j.bpc.2006.06.009. Epub 2006 Jun 22.
10
Comparative thermodynamics for monomer and dimer sequence-dependent binding of a heterocyclic dication in the DNA minor groove.DNA小沟中杂环双阳离子单体和二聚体序列依赖性结合的比较热力学
J Mol Biol. 2002 Mar 29;317(3):361-74. doi: 10.1006/jmbi.2002.5433.
引用本文的文献
1
The Impact of Using Different Cationic Polymers on the Formation of Efficient Lipopolyplexes for pDNA Delivery.使用不同阳离子聚合物对形成用于递送质粒DNA的高效脂质多聚体的影响。
Int J Nanomedicine. 2025 Aug 19;20:10021-10041. doi: 10.2147/IJN.S513568. eCollection 2025.
2
Revealing two distinct molecular binding modes in polyethyleneimine-DNA polyplexes using infrared spectroscopy.利用红外光谱揭示聚乙烯亚胺- DNA 多聚体中的两种不同分子结合模式。
Soft Matter. 2025 May 28;21(21):4192-4200. doi: 10.1039/d5sm00213c.
3
Hybrid fibrous architectures-mediated gene transfer by pDNA nanoparticles into macrophages.混合纤维结构介导的质粒DNA纳米颗粒向巨噬细胞的基因转移。
Heliyon. 2024 Sep 25;10(19):e38071. doi: 10.1016/j.heliyon.2024.e38071. eCollection 2024 Oct 15.
4
Closing the Gap between Experiment and Simulation─A Holistic Study on the Complexation of Small Interfering RNAs with Polyethylenimine.弥合实验与模拟之间的差距——聚亚乙基亚胺与小干扰 RNA 复合物的整体研究
Mol Pharm. 2024 May 6;21(5):2163-2175. doi: 10.1021/acs.molpharmaceut.3c00747. Epub 2024 Feb 19.
5
Structural DNA nanotechnology at the nexus of next-generation bio-applications: challenges and perspectives.处于下一代生物应用核心的结构DNA纳米技术：挑战与展望
Nanoscale Adv. 2023 Dec 19;6(2):386-401. doi: 10.1039/d3na00692a. eCollection 2024 Jan 16.
6
Inhibitors of Polyphosphate and Neutrophil Extracellular Traps.多聚磷酸盐和中性粒细胞胞外诱捕网抑制剂。
Semin Thromb Hemost. 2024 Oct;50(7):970-977. doi: 10.1055/s-0043-1768936. Epub 2023 May 16.
7
Antibiofilm Activity of PEGylated Branched Polyethylenimine.聚乙二醇化支链聚乙烯亚胺的抗生物膜活性
ACS Omega. 2022 Dec 2;7(49):44825-44835. doi: 10.1021/acsomega.2c04911. eCollection 2022 Dec 13.
8
Experimental study of a nanoscale translocation ratchet.纳米尺度 translocation 棘轮的实验研究。
Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2202527119. doi: 10.1073/pnas.2202527119. Epub 2022 Jul 18.
9
Laser-Assisted Direct Grafting of Poly(ethyleneimine) on Poly(methyl methacrylate).聚（乙烯亚胺）在聚（甲基丙烯酸甲酯）上的激光辅助直接接枝
Polymers (Basel). 2022 May 17;14(10):2041. doi: 10.3390/polym14102041.
10
The biological applications of DNA nanomaterials: current challenges and future directions.DNA 纳米材料的生物学应用：当前的挑战和未来的方向。
Signal Transduct Target Ther. 2021 Oct 8;6(1):351. doi: 10.1038/s41392-021-00727-9.
本文引用的文献
1
Amide spacing influences pDNA binding of poly(amidoamine)s.酰胺间距影响聚（酰胺-胺）与 pDNA 的结合。
Biomacromolecules. 2010 Feb 8;11(2):326-32. doi: 10.1021/bm900824g.
2
Visualization of the detailed structure of plasmid DNA.
J Phys Chem B. 2008 Dec 25;112(51):16788-92. doi: 10.1021/jp804634s.
3
Correlation of amine number and pDNA binding mechanism for trehalose-based polycations.基于海藻糖的聚阳离子的胺值与质粒DNA结合机制的相关性
Langmuir. 2008 Aug 5;24(15):8090-101. doi: 10.1021/la800120q. Epub 2008 Jul 8.
4
Controlled complexation of plasmid DNA with cationic polymers: effect of surfactant on the complexation and stability of the complexes.质粒DNA与阳离子聚合物的可控络合：表面活性剂对络合物络合及稳定性的影响。
Colloids Surf B Biointerfaces. 2008 Oct 1;66(1):77-83. doi: 10.1016/j.colsurfb.2008.05.012. Epub 2008 May 24.
5
Thermodynamics of DNA condensation caused by mn2+ binding.
Chem Pharm Bull (Tokyo). 2008 Mar;56(3):247-9. doi: 10.1248/cpb.56.247.
6
Deciphering the role of hydrogen bonding in enhancing pDNA-polycation interactions.解析氢键在增强质粒DNA-聚阳离子相互作用中的作用。
Langmuir. 2007 Sep 11;23(19):9773-84. doi: 10.1021/la7009995. Epub 2007 Aug 18.
7
Development of a fitting model suitable for the isothermal titration calorimetric curve of DNA with cationic ligands.
J Phys Chem B. 2006 Jun 8;110(22):10919-25. doi: 10.1021/jp057554e.
8
Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention.聚乙烯亚胺-DNA复合物聚集的机制研究及其预防
Biotechnol Bioeng. 2005 Jun 5;90(5):614-20. doi: 10.1002/bit.20444.
9
Contribution of hydrophobicity to thermodynamics of ligand-DNA binding and DNA collapse.疏水性对配体 - DNA 结合及 DNA 塌缩热力学的贡献。
Biophys J. 2005 Mar;88(3):2089-103. doi: 10.1529/biophysj.104.052100. Epub 2005 Jan 14.
10
Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis.探索聚乙烯亚胺介导的DNA转染及质子海绵假说。
J Gene Med. 2005 May;7(5):657-63. doi: 10.1002/jgm.696.
Zotero 插件