Department of Life Sciences, University of Trieste, Italy.
Acta Biomater. 2011 Jan;7(1):337-46. doi: 10.1016/j.actbio.2010.07.024. Epub 2010 Jul 23.
Bisphenol A glycidylmethacrylate (BisGMA)/triethyleneglycol dimethacrylate (TEGDMA) thermosets are receiving increasing attention as biomaterials for dental and orthopedic applications; for both these fields, bacterial adhesion to the surface of the implant represents a major issue for the outcome of the surgical procedure. Moreover, the biological behaviour of these materials is influenced by their ability to establish proper interactions between their surface and the eukaryotic cells of the surrounding tissues, which is important for good implant integration. The aim of this work was to develop an antimicrobial non-cytotoxic coating for methacrylic thermosets by means of a nanocomposite material based on a lactose-modified chitosan and antibacterial silver nanoparticles. The coating was characterized by UV-vis spectrophotometry, optical microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). In vitro tests were employed for a biological characterization of the material: antimicrobial efficacy tests were carried out with both Gram+ and Gram- strains. Osteoblast-like cell-lines, primary human fibroblasts and adipose-derived stem cells, were used for LDH cytotoxicity assays and Alamar blue cell proliferation assays. Cell morphology and distribution were evaluated by SEM and confocal laser scanning microscopy. In vitro results showed that the nanocomposite coating is effective in killing both bacterial strains and that this material does not exert any significant cytotoxic effect towards tested cells, which are able to firmly attach and proliferate on the surface of the coating. Such biocompatible antimicrobial polymeric films containing silver nanoparticles may have good potential for surface modification of medical devices, especially for prosthetic applications in orthopedics and dentistry.
双酚 A 缩水甘油甲基丙烯酸酯(BisGMA)/三乙二醇二甲基丙烯酸酯(TEGDMA)热固性树脂作为牙科和矫形应用的生物材料受到越来越多的关注;对于这两个领域,细菌在植入物表面的粘附是手术结果的一个主要问题。此外,这些材料的生物学行为受到其表面与周围组织的真核细胞之间建立适当相互作用的能力的影响,这对于良好的植入物整合很重要。本工作的目的是通过基于乳糖修饰壳聚糖和抗菌银纳米粒子的纳米复合材料来开发用于甲基丙烯酸酯热固性树脂的抗菌非细胞毒性涂层。通过紫外可见分光光度法、光学显微镜、透射电子显微镜(TEM)和扫描电子显微镜(SEM)对涂层进行了表征。体外试验用于对材料进行生物学特性表征:采用革兰氏阳性和革兰氏阴性菌株进行了抗菌功效测试。成骨样细胞系、原代人成纤维细胞和脂肪源性干细胞用于 LDH 细胞毒性测定和 Alamar 蓝细胞增殖测定。通过 SEM 和共聚焦激光扫描显微镜评估细胞形态和分布。体外结果表明,纳米复合涂层可有效杀灭两种细菌菌株,并且该材料对测试细胞没有任何显著的细胞毒性作用,测试细胞能够牢固地附着并在涂层表面增殖。这种含有银纳米粒子的生物相容型抗菌聚合物薄膜可能具有用于医疗器械表面改性的良好潜力,特别是在矫形和牙科中的假体应用。