American Red Cross Blood Services, Southern California Region, Pomona, CA, USA.
Transfus Med Rev. 2010 Jul;24(3):172-94. doi: 10.1016/j.tmrv.2010.03.001.
Flow cytometry operators often apply familiar white blood cell (WBC) methods when studying red blood cell (RBC) antigens and antibodies. Some WBC methods are not appropriate for RBCs, as the analysis of RBCs requires special considerations, for example, avoidance of agglutination. One hundred seventy-six published articles from 88 groups studying RBC interactions were reviewed. Three fourths of groups used at least one unnecessary WBC procedure for RBCs, and about one fourth did not use any method to prevent/disperse RBC agglutination. Flow cytometric studies were performed to determine the effect of RBC agglutination on results and compare different methods of preventing and/or dispersing agglutination. The presence of RBC agglutinates have been shown to be affected by the type of pipette tip used for mixing RBC suspensions, the number of antigen sites/RBC, the type and concentration of primary antibody, and the type of secondary antibody. For quantitation methods, for example, fetal maternal hemorrhage, the presence of agglutinates have been shown to adversely affect results (fewer fetal D+ RBCs detected).
流式细胞术操作人员在研究红细胞（RBC）抗原和抗体时，通常会应用熟悉的白细胞（WBC）方法。有些 WBC 方法不适合 RBC，因为 RBC 的分析需要特殊考虑，例如避免凝集。本文综述了 88 个研究 RBC 相互作用的小组的 176 篇已发表的文章。四分之三的小组对 RBC 使用了至少一种不必要的 WBC 程序，约四分之一的小组没有使用任何方法来防止/分散 RBC 凝集。进行流式细胞术研究是为了确定 RBC 凝集对结果的影响，并比较防止和/或分散凝集的不同方法。已经表明，用于混合 RBC 悬浮液的吸管尖端的类型、抗原位点/RBC 的数量、一抗的类型和浓度以及二抗的类型都会影响 RBC 聚集物的存在。例如，对于定量方法，胎儿母血丢失，聚集物的存在已被证明会对结果产生不利影响（检测到的胎儿 D+ RBC 较少）。
