Nagel R, Bresch H, Caspers N, Hansen P D, Markert M, Munk R, Scholz N, ter Höfte B B
Institut für Zoologie, Johannes Gutenberg-Universität Mainz, Germany.
Ecotoxicol Environ Saf. 1991 Apr;21(2):157-64. doi: 10.1016/0147-6513(91)90017-j.
An early life-stage (ELS) test was conducted with the zebrafish and 3,4-dichloroaniline (3,4-DCA) in eight laboratories. Based on the results from all eight laboratories, an LOEC of 200 micrograms 3,4-dichloroaniline/liter applies for the early life stages of the zebrafish. Effects observed were reduction of the survival rate and malformations. If the 100 micrograms/liter concentration additionally tested by one laboratory is included in the assessment, an LOEC of 100 micrograms 3,4-DCA/liter is obtained for the survival rate and increase in length. The NOEC is 20 micrograms/liter. The present results of a comparative laboratory study with the zebrafish show that a 42-day ELS test can be conducted with this species of fish, and affords meaningful results.
