Czuba M
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Ecotoxicol Environ Saf. 1991 Apr;21(2):194-206. doi: 10.1016/0147-6513(91)90021-g.
Meristematic cells of carrot (Daucus carota--Ca68-10) were grown in a heterogeneous suspension culture in the absence of (2,4-dichlorophenoxy)acetic acid in 71-V medium in the dark. Freshly inoculated cell cultures were treated daily with methyl mercury for the first 4 days and harvested on Day 7. The cultures were separated (by selective filtration) into groups of aggregates varying in size from 44 to 900 microns. Methyl mercury severely decreased the cell mass of the dominant (300-microns) fraction. When separate aggregates were cultured (44 to 900 microns) and treated as before, the inhibition of the 300 microns fraction was more evident. Separately cultured cell groups of 200- or 300-microns size developed into larger heterogeneous populations and contained the dominant 300-microns size group which was the most sensitive to methyl mercury. Other cell groups (900-, 500-, and 44-microns) failed to produce a large heterogeneous cell population when cultured separately. Without the 300-microns-sized aggregate, neither the whole culture nor the separate aggregates culture (900, 500, or 44 microns) could survive, suggesting the "nurse" cell role of the 300-microns cell group.
胡萝卜（胡萝卜——Ca68 - 10）的分生细胞在黑暗中于71 - V培养基中、在无（2,4 - 二氯苯氧基）乙酸的情况下进行异质悬浮培养。在最初4天，每天用甲基汞处理新接种的细胞培养物，并在第7天收获。通过选择性过滤将培养物分离成大小从44微米到900微米不等的聚集体组。甲基汞严重降低了占主导的（300微米）部分的细胞量。当将单独的聚集体（44微米至900微米）进行培养并按之前的方式处理时，对300微米部分的抑制更为明显。单独培养的200微米或300微米大小的细胞组发展成更大的异质群体，并包含对甲基汞最敏感的占主导的300微米大小的组。其他细胞组（900微米、500微米和44微米）单独培养时未能产生大量异质细胞群体。没有300微米大小的聚集体，整个培养物或单独的聚集体培养物（900微米、500微米或44微米）都无法存活，这表明300微米细胞组具有“滋养”细胞的作用。
