Czuba M
Ecotoxicol Environ Saf. 1987 Apr;13(2):191-201. doi: 10.1016/0147-6513(87)90007-8.
Cultures of Daucus carota, Ca-68-10, and Lactuca sativa, Le-67, were grown at increasing methyl mercury (MeHg) concentrations ranging from initial doses of 0.05 to 5.0 micrograms/ml per day for 4 days with or without 0.15 microgram/ml 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence or absence of light. The presence of 2,4-D interacted with light synergistically in the expression of MeHg toxicity within the whole range of concentrations. Demethylation patterns increased or decreased depending on the species, the 2,4-D concentration in the medium, and methyl mercury concentration used in the treatment. Lettuce was more sensitive to this interaction than carrot. In lettuce, the presence of 2,4-D in the light lowered the concentration of total Hg (or MeHg) required to reduce growth by 50%, about 13 times relative to that in the dark (i.e., it sensitized the cells). In the absence of 2,4-D the pattern was reversed. In carrot the pattern was similar but less pronounced. This suggests that, in these cell populations, MeHg toxicity is partly a hormone-mediated and light-sensitive event.
将胡萝卜（Ca - 68 - 10）和生菜（Le - 67）的培养物，在有或无光照的条件下，于甲基汞（MeHg）浓度逐渐增加（初始剂量为每天0.05至5.0微克/毫升）的环境中培养4天，培养基中添加或不添加0.15微克/毫升的2,4 - 二氯苯氧乙酸（2,4 - D）。在整个浓度范围内，2,4 - D的存在与光照在MeHg毒性表达方面具有协同作用。脱甲基化模式根据物种、培养基中2,4 - D的浓度以及处理中使用的甲基汞浓度而增加或减少。生菜对这种相互作用比胡萝卜更敏感。在生菜中，光照下2,4 - D的存在降低了使生长减少50%所需的总汞（或MeHg）浓度，相对于黑暗条件下约为13倍（即它使细胞更敏感）。在没有2,4 - D的情况下，模式相反。在胡萝卜中模式相似但不太明显。这表明，在这些细胞群体中，MeHg毒性部分是由激素介导且对光敏感的事件。
